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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Endy, Drew Resnekov, Orna Benjamin, Kirsten R. Love, Tonya Pincus, David Bush, Alan Brent, Roger Gordon, Andrew Yu, Richard C. Thomson, Ty M. Colman-lerner, Alejandro |
| Description | Author Affiliation: Thomson TM ( Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.); |
| Abstract | Although the proteins comprising many signaling systems are known, less is known about their numbers per cell. Existing measurements often vary by more than 10-fold. Here, we devised improved quantification methods to measure protein abundances in the Saccharomyces cerevisiae pheromone response pathway, an archetypical signaling system. These methods limited variation between independent measurements of protein abundance to a factor of two. We used these measurements together with quantitative models to identify and investigate behaviors of the pheromone response system sensitive to precise abundances. The difference between the maximum and basal signaling output (dynamic range) of the pheromone response MAPK cascade was strongly sensitive to the abundance of Ste5, the MAPK scaffold protein, and absolute system output depended on the amount of Fus3, the MAPK. Additional analysis and experiment suggest that scaffold abundance sets a tradeoff between maximum system output and system dynamic range, a prediction supported by recent experiments. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 50 |
| Volume Number | 108 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2011-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Saccharomyces Cerevisiae Proteins Metabolism Saccharomyces Cerevisiae Signal Transduction Systems Biology Fluorescence Immunoblotting MAP Kinase Signaling System Models, Biological Pheromones Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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