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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Budelli, Gonzalo Geng, Yanyan Butler, Alice Magleby, Karl L. Salkoff, Lawrence |
| Description | Author Affiliation: Budelli G ( Departments of Anatomy and Neurobiology and Genetics, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.); |
| Abstract | High-conductance $Ca^{2+}-$ and voltage-activated $K^{+}$ (Slo1 or BK) channels (KCNMA1) play key roles in many physiological processes. The structure of the Slo1 channel has two functional domains, a core consisting of four voltage sensors controlling an ion-conducting pore, and a larger tail that forms an intracellular gating ring thought to confer $Ca^{2+}$ and $Mg^{2+}$ sensitivity as well as sensitivity to a host of other intracellular factors. Although the modular structure of the Slo1 channel is known, the functional properties of the core and the allosteric interactions between core and tail are poorly understood because it has not been possible to study the core in the absence of the gating ring. To address these questions, we developed constructs that allow functional cores of Slo1 channels to be expressed by replacing the 827-amino acid gating ring with short tails of either 74 or 11 amino acids. Recorded currents from these constructs reveals that the gating ring is not required for either expression or gating of the core. Voltage activation is retained after the gating ring is replaced, but all $Ca^{2+}-$ and $Mg^{2+}-dependent$ gating is lost. Replacing the gating ring also right-shifts the conductance-voltage relation, decreases mean open-channel and burst duration by about sixfold, and reduces apparent mean single-channel conductance by about 30%. These results show that the gating ring is not required for voltage activation but is required for $Ca^{2+}$ and $Mg^{2+}$ activation. They also suggest possible actions of the unliganded (passive) gating ring or added short tails on the core. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 41 |
| Volume Number | 110 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2013-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Ion Channel Gating Physiology Kv1.4 Potassium Channel Chemistry Metabolism Large-Conductance Calcium-Activated Potassium Channel Alpha Subunits Animals Calcium Drug Effects Kinetics Antagonists & Inhibitors Genetics Magnesium Mice Mutagenesis, Site-Directed Oligonucleotides Oocytes Patch-Clamp Techniques Peptides Pharmacology Tetraethylammonium Xenopus Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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