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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dhungel, Kshitiz Upadhyay Kim, Tae Woon Sharma, Naveen Bhattarai, Janardhan Prasad Park, Seon Ah Han, Seong Kyu Kim, Chul Jin |
| Description | Author Affiliation: Dhungel KU ( Research Institute of Clinical Medicine, Chonbuk National University Medical School & Hospital, Jeonju, Korea.) |
| Abstract | Objectives: Magnesium has been known for treating vasospasm following subarachnoid hemorrhage. However, its action mechanism in cerebral vascular relaxation is not clear. Potassium channels play a pivotal role in the relaxation of smooth muscle cells. To investigate their role in magnesium-induced relaxation of basilar smooth muscle cells, we examined the effect of magnesium on potassium channels using the patch clamp technique on acutely isolated smooth muscle cells from rabbit basilar artery. Method: Fresh smooth muscle cells were isolated from the basilar artery by enzyme treatment. To identify which potassium channels are involved in the magnesium-induced currents, we used the potassium channel blockers tetraethylammonium (TEA), glibenclamide, apamin and iberiotoxin (IBX). Results: Magnesium (5 mM) increased the step pulse-induced outward $K^{+}$ currents by 46% over control level (P<0·01). The outward $K^{+}$ current was decreased to 22% (P<0·01) by TEA (10 mM), a non-specific $K^{+}$ channel blocker, and to 60% of control level (P<0·01) by IBX (0·1 μM,), a large-conductance $Ca^{2+}-activated$ $K^{+}$ (BK) channel blocker, but was not inhibited by apamin (1 nM), a small-conductance $Ca^{2+}-activated$ potassium (SK) channel blocker, or glibenclamide (3 mM), an adenosine triphosphate (ATP)-sensitive $K^{+}$ channel blocker. Caffeine (3 mM) enhanced outward $K^{+}$ currents. Magnesium-induced increase of outward $K^{+}$ currents persisted in the presence of apamin. However, magnesium failed to increase the outward $K^{+}$ currents in the presence of IBX. Discussion: These results demonstrate that BK channels are functionally expressed in rabbit basilar smooth muscle cells and suggest that BK channels may play a pivotal role in magnesium-induced relaxation. |
| File Format | HTM / HTML |
| ISSN | 01616412 |
| e-ISSN | 17431328 |
| Journal | Neurological Research |
| Issue Number | 1 |
| Volume Number | 34 |
| Language | English |
| Publisher | Taylor & Francis |
| Publisher Date | 2012-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Neurology Large-conductance Calcium-activated Potassium Channels Metabolism Magnesium Pharmacology Muscle, Smooth, Vascular Physiology Peptides Potassium Channel Blockers Animals Apamin Basilar Artery Drug Effects Calcium Cells, Cultured Glyburide Muscle Relaxation Patch-clamp Techniques Potassium Potassium Channels Rabbits Tetraethylammonium |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neurology Neurology (clinical) |
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