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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Horwitz, Joseph Hubbell, Wayne L. López, Carlos J. Yang, Zhongyu Kreitman, Margaux J. Lerch, Michael T. |
| Description | Author Affiliation: Lerch MT ( Jules Stein Eye Institute, University of California, Los Angeles, CA 90095); López CJ ( Jules Stein Eye Institute, University of California, Los Angeles, CA 90095); Yang Z ( Jules Stein Eye Institute, University of California, Los Angeles, CA 90095); Kreitman MJ ( Jules Stein Eye Institute, University of California, Los Angeles, CA 90095); Horwitz J ( Jules Stein Eye Institute, University of California, Los Angeles, CA 90095); Hubbell WL ( Jules Stein Eye Institute, University of California, Los Angeles, CA 90095); |
| Abstract | Application of hydrostatic pressure shifts protein conformational equilibria in a direction to reduce the volume of the system. A current view is that the volume reduction is dominated by elimination of voids or cavities in the protein interior via cavity hydration, although an alternative mechanism wherein cavities are filled with protein side chains resulting from a structure relaxation has been suggested [López CJ, Yang Z, Altenbach C, Hubbell WL (2013) Proc Natl Acad Sci USA 110(46):E4306-E4315]. In the present study, mechanisms for elimination of cavities under high pressure are investigated in the L99A cavity mutant of T4 lysozyme and derivatives thereof using site-directed spin labeling, pressure-resolved double electron-electron resonance, and high-pressure circular dichroism spectroscopy. In the L99A mutant, the ground state is in equilibrium with an excited state of only â ¼ 3% of the population in which the cavity is filled by a protein side chain [Bouvignies et al. (2011) Nature 477(7362):111-114]. The results of the present study show that in L99A the native ground state is the dominant conformation to pressures of 3 kbar, with cavity hydration apparently taking place in the range of 2-3 kbar. However, in the presence of additional mutations that lower the free energy of the excited state, pressure strongly populates the excited state, thereby eliminating the cavity with a native side chain rather than solvent. Thus, both cavity hydration and structure relaxation are mechanisms for cavity elimination under pressure, and which is dominant is determined by details of the energy landscape. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 19 |
| Volume Number | 112 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2015-05-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacteriophage T4 Enzymology Muramidase Chemistry Mutation Circular Dichroism Electron Spin Resonance Spectroscopy Hydrogen-Ion Concentration Hydrostatic Pressure Ligands Magnetic Resonance Spectroscopy Models, Molecular Genetics Mutagenesis, Site-Directed Protein Denaturation Protein Folding Protein Structure, Secondary Solvents Structure-Activity Relationship Temperature Thermodynamics Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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