| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Akagi, Keiko Symer, David E. Li, Wei Liyanarachchi, Sandya Wu, Dayong Wang, Qianben Wang, Yao Phay, John De La Chapelle, Albert He, Huiling Nagy, Rebecca Wang, Yanqiang Srinivas, Mukund Jin, Victor Shen, Rulong |
| Description |
Author Affiliation: He H ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics, huiling.he@osumc.edu albert.delachapelle@osumc.edu.); Li W ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics.); Liyanarachchi S ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics.); Srinivas M ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics.); Wang Y ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics.); Akagi K ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics.); Wang Y ( Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78249.); Wu D ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics.); Wang Q ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics.); Jin V ( Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78249.); Symer DE ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics, Department of Biomedical Informatics, Department of Internal Medicine.); Shen R ( Department of Pathology, and.); Phay J ( Department of Surgery, The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210); Nagy R ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics, Department of Internal Medicine.); de la Chapelle A ( Human Cancer Genetics Program and Department of Molecular Virology, Immunology, and Medical Genetics, huiling.he@osumc.edu albert.delachapelle@osumc.edu.); |
| Abstract |
The [A] allele of SNP rs965513 in 9q22 has been consistently shown to be highly associated with increased papillary thyroid cancer (PTC) risk with an odds ratio of â ¼1.8 as determined by genome-wide association studies, yet the molecular mechanisms remain poorly understood. Previously, we noted that the expression of two genes in the region, forkhead box E1 (FOXE1) and PTC susceptibility candidate 2 (PTCSC2), is regulated by rs965513 in unaffected thyroid tissue, but the underlying mechanisms were not elucidated. Here, we fine-mapped the 9q22 region in PTC and controls and detected an â ¼33-kb linkage disequilibrium block (containing the lead SNP rs965513) that significantly associates with PTC risk. Chromatin characteristics and regulatory element signatures in this block disclosed at least three regulatory elements functioning as enhancers. These enhancers harbor at least four SNPs (rs7864322, rs12352658, rs7847449, and rs10759944) that serve as functional variants. The variant genotypes are associated with differential enhancer activities and/or transcription factor binding activities. Using the chromosome conformation capture methodology, long-range looping interactions of these elements with the promoter region shared by FOXE1 and PTCSC2 in a human papillary thyroid carcinoma cell line (KTC-1) and unaffected thyroid tissue were found. Our results suggest that multiple variants coinherited with the lead SNP and located in long-range enhancers are involved in the transcriptional regulation of FOXE1 and PTCSC2 expression. These results explain the mechanism by which the risk allele of rs965513 predisposes to thyroid cancer. |
| ISSN |
00278424 |
| e-ISSN |
10916490 |
| Journal |
Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number |
19 |
| Volume Number |
112 |
| Language |
English |
| Publisher |
National Academy of Sciences |
| Publisher Date |
2015-05-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Carcinoma Genetics Enhancer Elements, Genetic Genome-Wide Association Study Polymorphism, Single Nucleotide Thyroid Neoplasms Alleles Cell Line, Tumor Chromatin Chemistry Chromatin Immunoprecipitation Forkhead Transcription Factors Genetic Predisposition To Disease Genotype Haplotypes Histones Odds Ratio Penetrance Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Multidisciplinary |
