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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Strobel, Eric J. Roberts, Jeffrey W. |
| Description | Author Affiliation: Strobel EJ ( Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853.); Roberts JW ( Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853 jwr7@cornell.edu.); |
| Abstract | The movement of RNA polymerase (RNAP) during transcription elongation is modulated by DNA-encoded elements that cause the elongation complex to pause. One of the best-characterized pause sequences is a binding site for the σ(70) initiation factor that induces pausing at a site near lambdoid phage late-gene promoters. An essential component of this σ(70)-dependent pause is the elemental pause site (EPS), a sequence that itself induces transcription pausing throughout the Escherichia coli genome and underlies other complex regulatory pause elements, such as the ops and his operon pauses. Here, we identify and provide a detailed kinetic analysis of a transcription cycle analogous to abortive cycling that underlies the σ(70)-dependent pause. We show that, in σ(70)-dependent pausing, the elemental pause acts primarily to modulate the rate at which complexes attempt to disengage the σ(70):DNA interaction. Our findings establish the σ(70)-dependent pause-encoding region as a multipartite element in which several pause-inducing components make distinct mechanistic contributions to the induction and maintenance of a regulatory transcription pause. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 32 |
| Volume Number | 112 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2015-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Escherichia Coli Genetics Promoter Regions, Genetic Sigma Factor Metabolism Transcription Elongation, Genetic Base Composition DNA, Bacterial Kinetics Models, Biological Molecular Sequence Data Mutation Nucleotides Probability RNA, Bacterial Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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