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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Runkel, Anne M. Brunkard, Jacob O. Zambryski, Patricia C. |
| Description | Author Affiliation: Brunkard JO ( Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720.); Runkel AM ( Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720.); Zambryski PC ( Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 zambrysk@berkeley.edu.); |
| Abstract | A fundamental mystery of plant cell biology is the occurrence of 'stromules,' stroma-filled tubular extensions from plastids (such as chloroplasts) that are universally observed in plants but whose functions are, in effect, completely unknown. One prevalent hypothesis is that stromules exchange signals or metabolites between plastids and other subcellular compartments, and that stromules are induced during stress. Until now, no signaling mechanisms originating within the plastid have been identified that regulate stromule activity, a critical missing link in this hypothesis. Using confocal and superresolution 3D microscopy, we have shown that stromules form in response to light-sensitive redox signals within the chloroplast. Stromule frequency increased during the day or after treatment with chemicals that produce reactive oxygen species specifically in the chloroplast. Silencing expression of the chloroplast NADPH-dependent thioredoxin reductase, a central hub in chloroplast redox signaling pathways, increased chloroplast stromule frequency, whereas silencing expression of nuclear genes related to plastid genome expression and tetrapyrrole biosynthesis had no impact on stromules. Leucoplasts, which are not photosynthetic, also made more stromules in the daytime. Leucoplasts did not respond to the same redox signaling pathway but instead increased stromule formation when exposed to sucrose, a major product of photosynthesis, although sucrose has no impact on chloroplast stromule frequency. Thus, different types of plastids make stromules in response to distinct signals. Finally, isolated chloroplasts could make stromules independently after extraction from the cytoplasm, suggesting that chloroplast-associated factors are sufficient to generate stromules. These discoveries demonstrate that chloroplasts are remarkably autonomous organelles that alter their stromule frequency in reaction to internal signal transduction pathways. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 32 |
| Volume Number | 112 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2015-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Chloroplasts Metabolism Signal Transduction Tobacco Benzoquinones Pharmacology Ultrastructure Circadian Rhythm Drug Effects Diuron Gene Silencing Green Fluorescent Proteins Models, Biological Molecular Sequence Data NADP Oxidation-Reduction Photosynthesis Phylogeny Plant Epidermis Plant Proteins Genetics Reactive Oxygen Species Sucrose Thioredoxin-Disulfide Reductase Time-Lapse Imaging Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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