NDLI: Optimization of Expression Conditions Enhances Production of Sepiapterin, a Precursor for Tetrahydrobiopterin Biosynthesis, in Recombinant Escherichia coli.

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Optimization of Expression Conditions Enhances Production of Sepiapterin, a Precursor for Tetrahydrobiopterin Biosynthesis, in Recombinant Escherichia coli.

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Optimization of Expression Conditions Enhances Production of Sepiapterin, a Precursor for Tetrahydrobiopterin Biosynthesis, in Recombinant Escherichia coli.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Park, Eun-Hee Lee, Won-Heong Jang, Mi-Hee Kim, Myoung-Dong
Description	Author Affiliation: Park EH ( Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.); Lee WH ( Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.); Jang MH ( Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.); Kim MD ( Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.)
Abstract	Sepiapterin is a precursor for the synthesis of tetrahydrobiopterin (BH4), which is a wellknown cofactor for aromatic amino acid hydroxylation and nitric oxide synthesis in higher mammals. In this study, a recombinant Escherichia coli BL21(DE3) strain harboring cyanobacterial guanosine 5'-triphosphate cyclohydrolase 1 (GCH1) and human 6- pyruvoyltetrahydropterin synthase (PTPS) genes was constructed to produce sepiapterin. The optimum conditions for T7 promoter-driven expression of GCH1 and PTPS were 30°C and 0.1 mM isopropyl-ß-D-thioglucopyranoside (IPTG). The maximum sepiapterin concentration of 88.1 ± 2.4 mg/l was obtained in a batch cultivation of the recombinant E. coli, corresponding to an 18-fold increase in sepiapterin production compared with the control condition (37°C and 1 mM IPTG).
File Format	HTM / HTML
ISSN	10177825
e-ISSN	17388872
Journal	Journal of Microbiology and Biotechnology
Issue Number	10
Volume Number	25
Language	English
Publisher	Korean Society for Microbiology and Biotechnology
Publisher Date	2015-10-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Biotechnology Biopterin Analogs & Derivatives Escherichia Coli Genetics Metabolism Metabolic Engineering Metabolic Networks And Pathways Pterins Biosynthesis Cyanobacteria Enzymology Gene Expression Temperature Transcriptional Activation Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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