NDLI: Real-Time Monitoring of Catheter-Related Biofilm Infection in Mice.

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Real-Time Monitoring of Catheter-Related Biofilm Infection in Mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liu, Xu Yin, Hong Xu, Xianxing Cheng, Yuanguo Cai, Yun Wang, Rui
Description	Author Affiliation: Liu X ( Department of Clinical Pharmacology, PLA General Hospital, Beijing 100853, P.R. China.); Yin H ( Traditional Chinese Medicine Pharmacy, PLA General Hospital, Beijing 100853, P.R. China.); Xu X ( Department of Pharmacy, the Second Artillery General Hospital, Beijing 100088, P.R. China.); Cheng Y ( State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P.R. China.); Cai Y ( Medicine Clinical Research Center, PLA General Hospital, Beijing 100853, P.R. China.); Wang R ( Medicine Clinical Research Center, PLA General Hospital, Beijing 100853, P.R. China.)
Abstract	This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BLI. The numbers of integrated brightness (IB) and viable bacterial count (VBC) in the biofilm disks in vitro were highest at 24 h after inoculation; the IB of biofilm in vivo was increased until 24 h after implantation. A statistical correlation was observed between IB and VBC in vitro by linear regression analysis. The actual VBC value in vivo can be estimated accurately by IB without sacrifice. In addition, we monitored the change in white blood cells (WBCs) during infection. The number of WBCs on day 7 was significantly higher in the infection group than in the control group. This study indicates that BLI is a simple, fast, and sensitive method to measure catheter biofilm infection in mice.
File Format	HTM / HTML
ISSN	10177825
e-ISSN	17388872
Journal	Journal of Microbiology and Biotechnology
Issue Number	10
Volume Number	25
Language	English
Publisher	Korean Society for Microbiology and Biotechnology
Publisher Date	2015-10-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Biotechnology Bacterial Load Biofilms Growth & Development Catheter-related Infections Microbiology Catheters Luminescent Measurements Pseudomonas Infections Pseudomonas Aeruginosa Physiology Animals Disease Models, Animal Leukocyte Count Mice Time Factors Evaluation Studies Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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