NDLI: Co-Localization of GABA Shunt Enzymes for the Efficient Production of Gamma-Aminobutyric Acid via GABA Shunt Pathway in Escherichia coli.

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Co-Localization of GABA Shunt Enzymes for the Efficient Production of Gamma-Aminobutyric Acid via GABA Shunt Pathway in Escherichia coli.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pham, Van Dung Somasundaram, Sivachandiran Park, Si Jae Lee, Seung Hwan Hong, Soon Ho
Description	Author Affiliation: Pham VD ( School of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.); Somasundaram S ( School of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.); Park SJ ( Department of Environmental Engineering and Energy, Myongji University, Yongin 17058, Republic of Korea.); Lee SH ( Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea.); Hong SH ( School of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.)
Abstract	Gamma-aminobutyric acid (GABA) is a non-protein amino acid, which is an important inhibitor of neurotransmission in the human brain. GABA is also used as the precursor of biopolymer Nylon-4 production. In this study, the carbon flux from the tricarboxylic acid cycle was directed to the GABA shunt pathway for the production of GABA from glucose. The GABA shunt enzymes succinate-semialdehyde dehydrogenase (GabD) and GABA aminotransferase (GabT) were co-localized along with the GABA transporter (GadC) by using a synthetic scaffold complex. The co-localized enzyme scaffold complex produced 0.71 g/l of GABA from 10 g/l of glucose. Inactivation of competing metabolic pathways in mutant E. coli strains XBM1 and XBM6 increased GABA production 13% to reach 0.80 g/l GABA by the enzymes co-localized and expressed in the mutant strains. The recombinant E. coli system developed in this study demonstrated the possibility of the pathway of the GABA shunt as a novel GABA production pathway.
File Format	HTM / HTML
ISSN	10177825
e-ISSN	17388872
Journal	Journal of Microbiology and Biotechnology
Issue Number	4
Volume Number	26
Language	English
Publisher	Korean Society for Microbiology and Biotechnology
Publisher Date	2016-04-28
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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