| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Yang, Jing Zhao, Jing-Xiang Wang, Ying Chen, Gan Cheng, Wei-Na Luo, Xin Pei, Xue-Tao Zhao, Lian Su, Qin Zhou, Hong |
| Description |
Author Affiliation: Yang J ( a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.); Zhao JX ( a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.); Wang Y ( a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.); Chen G ( a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.); Cheng WN ( b Department of Biological Engineering , College of Environment and Chemical Engineering, Yanshan University , Qinhuangdao , P. R. China.); Luo X ( a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.); Pei XT ( a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.); Zhao L ( a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.); Su Q ( c The First Affiliated Hospital of General Hospital of PLA , Beijing , P. R. China.); Zhou H ( a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.) |
| Abstract |
OBJECTIVE: To examine the effects of sodium pyruvate (SP) on metabolic acidosis. METHODS: For the in vivo experiments, we evaluated effects of SP on an ammonium chloride (NH4Cl)-induced hyperchloremic acidosis rat model. SP was infused at overall doses of 2, 4, and 6 mmol·kg(- 1) for the SP1, SP2, and SP3 groups, respectively. Treatment with sodium bicarbonate (SB) was used as a positive control (2 mmol·kg(- 1)), and treatment with normal saline (NS) was used as a volume control (2 mL·kg(- 1)). Blood was sampled from the ophthalmic venous plexus for pH, blood gases, electrolytes, glucose, creatinine (Cr), and urea analysis after injection. For the in vitro experiment, propionate was applied to induce intracellular acidosis in human endothelial cells. Intracellular pH (pHi) was fluorimetrically measured after the addition of SP. RESULTS: In the in vivo study, the pH of SP1 group showed no significant difference compared with that of the NS group. The SP2 and SP3 groups had a higher pH than the NS group (P < 0.01). The SP3 group had a higher pH than the SB group (P < 0.05) and SP1 group (P < 0.05). Moreover, SP treatment ameliorated the abnormality of calcium and decreased the blood potassium levels. The SP3 group had higher glucose levels than SP1 group (P < 0.05). No significant differences were observed between all the groups in the plasma Cr and urea levels. In the in vitro study, the pHi increased immediately after the addition of SP. CONCLUSION: The data suggest that intravascular treatment with SP represents a novel therapeutic strategy to ameliorate metabolic acidosis. |
| File Format |
HTM / HTML |
| ISSN |
21691401 |
| Issue Number |
1 |
| Volume Number |
44 |
| e-ISSN |
2169141X |
| Journal |
Artificial Cells, Nanomedicine, and Biotechnology |
| Language |
English |
| Publisher |
Taylor & Francis |
| Publisher Date |
2016-01-01 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
Subscribed |
| Subject Keyword |
Discipline Nanotechnology Discipline Biotechnology Acidosis Blood Drug Therapy Endothelial Cells Drug Effects Pyruvic Acid Pharmacology Acid-base Equilibrium Chemically Induced Pathology Ammonium Chloride Animals Blood Gas Analysis Blood Glucose Metabolism Calcium Cell Line Creatinine Humans Hydrogen-ion Concentration Male Potassium Rats Rats, Wistar Sodium Sodium Bicarbonate Urea Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Medicine Biomedical Engineering Biotechnology Pharmaceutical Science |
