NDLI: Prime-boost vaccination with plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cao, Shinuo Mousa, Ahmed Abdelmoniem Aboge, Gabriel Oluga Kamyingkird, Ketsarin Zhou, Mo Moumouni, Paul Franck Adjou Terkawi, Mohamad Alaa Masatani, Tatsunori Nishikawa, Yoshifumi Suzuki, Hiroshi Fukumoto, Shinya Xuan, Xuenan
Description	Country affiliation: Japan Author Affiliation: Cao S ( National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan.)
Abstract	A heterologous prime-boost vaccination regime with DNA and recombinant vaccinia virus (rvv) vectors expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In this study, we describe the effectiveness of the prime-boost strategy by immunizing dogs with a recombinant plasmid followed by vaccinia virus, both of which expressed the glutamic acid-rich protein (BgGARP) of Babesia gibsoni. The dogs immunized with the prime-boost regime developed a significantly high level of specific antibodies against BgGARP when compared with the control groups. The antibody level was strongly increased after a booster immunization with a recombinant vaccinia virus. Two weeks after the booster immunization with a recombinant vaccinia virus expressing BgGARP, the dogs were challenged with B. gibsoni parasite. The dogs immunized with the prime-boost regime showed partial protection, manifested as a significantly low level of parasitemia. These results indicated that this type of DNA/rvv prime-boost immunization approach may have use against B. gibsoni infection in dogs.
File Format	HTM / HTML
ISSN	12302821
Issue Number	4
Volume Number	58
e-ISSN	18961851
Journal	Acta Parasitologica
Language	English
Publisher	De Gruyter
Publisher Date	2013-12-01
Publisher Place	Poland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Parasitology Babesia Immunology Babesiosis Prevention & Control Dog Diseases Protozoan Vaccines Vaccination Methods Vaccines, Dna Animals Antibodies, Protozoan Blood Genetics Dogs Drug Carriers Female Parasitemia Administration & Dosage Treatment Outcome Vaccines, Synthetic Vaccinia Virus Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Parasitology

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