NDLI: Fasciola hepatica - the pilot study of in vitro assessing immune response against native and recombinant antigens of the fluke.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Baska, Piotr Zawistowska-Deniziak, Anna Zdziarska, Anna M. Wasyl, Katarzyna Wisniewski, Marcin Cywinska, Anna Klockiewicz, Maciej Januszkiewicz, Kamil Wedrychowicz, Halina
Description	Author Affiliation: Baska P ( Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland, piotr_baska@sggw.pl.)
Abstract	Fasciola hepatica is a liver fluke that infects 2.4 million of people and causes great economical loss in animal production. To date a 100% effective vaccine has not been developed and the disease is controlled by drug therapy. Great efforts are put into development of effective vaccine against parasite what is difficult since Fasciola spp. (like other helmints) during evolutionary process has developed sophisticated and efficient methods to evade immune response. During preliminary experiments it is convenient to use cell lines which are relatively cheap and allow for reproducible comparison of results between laboratories. We stimulated BOMA (bovine monocyte/macrophage cell line) and BOMAC (bovine macrophage cell line) with native or recombinant antigens of Fasciola hepatica and assessed IFN-Î³, IL-4 and TNF- level upon stimulation. We observed diminished secretion of proinflammatory TNF- in LPS activated BOMA cells stimulated with Excretory/Secretory products of adult fluke (Fh-ES). We also observed greater changes in gene expression in LPS activated BOMA cells than in non activated BOMA cells upon stimulation using Fh-ES. The results show possibility of using cell lines for in vitro research of bovine immune response against liver fluke, although this model still requires validation and further characterization.
File Format	HTM / HTML
ISSN	12302821
Issue Number	4
Volume Number	58
e-ISSN	18961851
Journal	Acta Parasitologica
Language	English
Publisher	De Gruyter
Publisher Date	2013-12-01
Publisher Place	Poland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Parasitology Antigens, Helminth Immunology Fasciola Hepatica Helminth Proteins Animals Genetics Cattle Cell Line Interferon-gamma Secretion Interleukin-4 Macrophages Monocytes Pilot Projects Recombinant Proteins Tumor Necrosis Factor-alpha Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Parasitology

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