NDLI: Effect of electrical stimulation of the LES on LES pressure in a canine model.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sanmiguel, Claudia P. Hagiike, Masanobu Mintchev, Martin P. Cruz, Ryan Dela Phillips, Edward H. Cunneen, Scott A. Conklin, Jeffrey L. Soffer, Edy E.
Description	Country affiliation: United States Author Affiliation: Sanmiguel CP ( Center for Digestive Diseases, GI Motility Program, 8730 Alden Dr., Thalians Bldg., 2nd floor East, Los Angeles, CA 90048, USA.)
Abstract	Gastric electrical stimulation modulates lower esophageal sphincter pressure (LESP). High-frequency neural stimulation (NES) can induce gut smooth muscle contractions. To determine whether lower esophageal sphincter (LES) electrical stimulation (ES) can affect LESP, bipolar electrodes were implanted in the LES of four dogs. Esophageal manometry during sham or ES was performed randomly on separate days. Four stimuli were used: 1) low-frequency: 350-ms pulses at 6 cycles/min; 2) high-frequency-1: 1-ms pulses at 50 Hz; 3) high-frequency-2: 1-ms pulses at 20 Hz; and 4) NES: 20-ms bipolar pulses at 50 Hz. Recordings were obtained postprandially. Tests consisted of three 20-min periods: baseline, stimulation/sham, and poststimulation. The effect of NES was tested under anesthesia and following IV administration of l-NAME and atropine. Area under the curve (AUC) and LESP were compared among the three periods, by ANOVA and t-test, P < 0.05. Data are shown as means +/- SD. We found that low-frequency stimulation caused a sustained increase in LESP: 32.1 +/- 12.9 (prestimulation) vs. 43.2 +/- 18.0 (stimulation) vs. 50.1 +/- 23.8 (poststimulation), P < 0.05. AUC significantly increased during and after stimulation. There were no significant changes with other types of ES. With NES, LESP initially rose and then decreased below baseline (LES relaxation). During NES, N(G)-nitro-l-arginine methyl ester increased both resting LESP and the initial rise in LESP and markedly diminished the relaxation. Atropine lowered resting LESP and abolished the initial rise in LESP. In conclusion, low frequency ES of the LES increases LESP in conscious dogs. NES has dual effect on LESP: an initial stimulation, cholinergically mediated, followed by relaxation mediated by nitric oxide.
File Format	HTM / HTML
ISSN	01931857
Issue Number	2
Volume Number	295
e-ISSN	15221547
Journal	AJP: Gastrointestinal and Liver Physiology
Language	English
Publisher	American Physiological Society
Publisher Date	2008-08-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Physiology Discipline Gastroenterology Esophageal Sphincter, Lower Physiology Animals Atropine Pharmacology Dogs Electric Stimulation Drug Effects Female Manometry Ng-nitroarginine Methyl Ester Pressure Journal Article
Content Type	Text
Resource Type	Article
Subject	Hepatology Physiology Physiology (medical) Gastroenterology

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