NDLI: Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kanaya, Takashi Miyazawa, Kohtaro Takakura, Ikuro Itani, Wataru Watanabe, Kouichi Ohwada, Shyuichi Kitazawa, Haruki Rose, Michael T. McConochie, Huw R. Okano, Hideyuki Yamaguchi, Takahiro Aso, Hisashi
Description	Country affiliation: Japan Author Affiliation: Kanaya T ( Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku Univ., 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555 Sendai, Japan.)
Abstract	M cells are a kind of intestinal epithelial cell in the follicle-associated epithelium of Peyer's patches. These cells can transport antigens and microorganisms into underlying lymphoid tissues. Despite the important role of M cells in mucosal immune responses, the origin and mechanisms of differentiation as well as cell death of M cells remain unclear. To clarify the mechanism of M cell differentiation, we established a novel murine intestinal epithelial cell line (MIE) from the C57BL/6 mouse. MIE cells grow rapidly and have a cobblestone morphology, which is a typical feature of intestinal epithelial cells. Additionally, they express cytokeratin, villin, cell-cell junctional proteins, and alkaline phosphatase activity and can form microvilli. Their expression of Musashi-1 antigen indicates that they may be close to intestinal stem cells or transit-amplifying cells. MIE cells are able to differentiate into the M cell lineage following coculture with intestinal lymphocytes, but not with Peyer's patch lymphocytes (PPL). However, PPL costimulated with anti-CD3/CD28 MAbs caused MIE cells to display typical features of M cells, such as transcytosis activity, the disorganization of microvilli, and the expression of M cell markers. This transcytosis activity of MIE cells was not induced by T cells isolated from PPL costimulated with the same MAbs and was reduced by the depletion of the T cell population from PPL. A mixture of T cells treated with MAbs and B cells both from PPL led MIE cells to differentiate into M cells. We report here that MIE cells have the potential ability to differentiate into M cells and that this differentiation required activated T cells and B cells.
File Format	HTM / HTML
ISSN	01931857
Issue Number	2
Volume Number	295
e-ISSN	15221547
Journal	AJP: Gastrointestinal and Liver Physiology
Language	English
Publisher	American Physiological Society
Publisher Date	2008-08-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Physiology Discipline Gastroenterology Cell Differentiation Physiology Cell Lineage Intestinal Mucosa Cytology Animals Cell Line Coculture Techniques Ultrastructure Male Mice Mice, Inbred Balb C Microscopy, Phase-contrast Peyer's Patches Specific Pathogen-free Organisms Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Hepatology Physiology Physiology (medical) Gastroenterology

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