NDLI: Effect of maturation on the expression of aquaporin 3 in mouse oocyte.

Please wait, while we are loading the content...

Year: 2016 Volume: 24

Year: 2015 Volume: 23

Year: 2014 Volume: 22

Year: 2013 Volume: 21

Year: 2012 Volume: 20

Year: 2011 Volume: 19

Year: 2011 Volume: 19 Issue: 4

Year: 2011 Volume: 19 Issue: 3

Year: 2011 Volume: 19 Issue: 2

Year: 2011 Volume: 19 Issue: 1

Quality of transgenic rabbit embryos with different EGFP gene constructs.

Effect of maturation on the expression of aquaporin 3 in mouse oocyte.

Time course of meiotic spindle development in MII oocytes.

Optimization of artificial fertilization in the stinging catfish Heteropneustes fossilis (Bloch).

Short-term treatment with 6-DMAP and demecolcine improves developmental competence of electrically or Thi/DTT-activated porcine parthenogenetic embryos.

Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish

Nek2 and its substrate, centrobin/Nip2, are required for proper meiotic spindle formation of the mouse oocytes.

Stability of housekeeping genes and expression of locally produced growth factors and hormone receptors in goat preantral follicles.

Multiple histone site epigenetic modifications in nuclear transfer and in vitro fertilized bovine embryos.

Review: role of tubal environment in preimplantation embryogenesis: application to co-culture assays.

Association of distributions of three types of germinal vesicle stage oocytes with the canine follicle location in the ovary.

Capacitation status of activated bovine sperm cultured in media containing methyl-ß-cyclodextrin affects the acrosome reaction and fertility.

Year: 2010 Volume: 18

Year: 2009 Volume: 17

Year: 2008 Volume: 16

Year: 2007 Volume: 15

Effect of maturation on the expression of aquaporin 3 in mouse oocyte.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jo, Jun Woo Jee, Byung Chul Suh, Chang Suk Kim, Seok Hyun Choi, Young Min Kim, Jung Gu Moon, Shin Yong
Description	Author Affiliation: Jo JW ( Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463-707, Korea.)
Abstract	This study aimed to investigate whether aquaporin 3 (Aqp3) mRNAs are expressed in immature oocytes and altered during in vitro maturation process. Five- to 6-week-old female ICR mice were primed by gonadotropin for 24 and 48 h. Immature oocytes obtained 48 h after priming were also matured in vitro for 17 to 18 h. In vivo matured oocytes were obtained after 48 h priming followed by hCG injection. Total RNAs were extracted from 80 to 150 oocytes in each experimental group, and the levels of Aqp3 mRNA were quantified by real-time reverse transcriptase polymerase chain reaction. The experiments were repeated twice using different oocytes. The Aqp3 mRNA was expressed in immature oocytes, as well as in in vitro and in vivo matured oocytes. The expression level was higher in immature oocytes obtained 48 h after priming (17.2 ± 8.6, mean ± SD) than those with no priming (5.7 ± 0.8) or obtained 24 h after priming (2.5 ± 0.8). The expression of Aqp3 mRNA decreased after in vitro maturation (1.2 ± 0.5), which was similar to in vivo matured oocytes (1.0 ± 0.0). Our work demonstrated that Aqp3 mRNA expression increased during the development of immature oocyte but decreased after completion of in vitro maturation. The results indicate that AQP3 is certainly needed for the acquisition of immature oocytes' full growing potential within antral follicles.
File Format	HTM / HTML
ISSN	09671994
Issue Number	1
Volume Number	19
e-ISSN	14698730
Journal	Zygote
Language	English
Publisher	Cambridge University Press
Publisher Date	2011-02-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Embryology Aquaporin 3 Genetics Oocytes Metabolism Animals Female Mice Mice, Inbred Icr Cytology Ovarian Follicle Drug Effects Rna, Messenger Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in