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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yoon, Sook-Young Choe, Changyong Kim, Eun-Jin Kim, Chang-Woon Han, Jaehee Kang, Dawon |
| Description | Author Affiliation: Yoon SY ( CHA Medical Center, CHA University, Seoul, Korea.) |
| Abstract | 1. In mice, acetylcholine (ACh) plays an important role in oocyte activation and embryonic development. However, the role of ACh in mouse oocyte maturation has not been investigated. 2. In the present study, the effects of 100 µmol/L and 1 mmol/L ACh on maturation processes of murine germinal vesicle (GV) intact oocytes (GV oocytes) exposed to 10 and 100 µmol/L 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of cyclic nucleotide phosphodiesterase, were evaluated morphologically and immunologically. It has been shown that IBMX inhibits the resumption of meiosis by preventing cAMP breakdown. 3. In the present study, at the start of in vitro culture 100% of oocytes were at the GV stage. After 18 h culture, 95 ± 3, 0 and 85.8 ± 10.2% of oocytes had passed the GV stage in the control, IBMX and IBMX + ACh groups, respectively. The IBMX-induced inhibition of the maturation process was significantly attenuated by approximately 90% by ACh in groups treated with 10 µmol/L IBMX + 100 µmol/L ACh and 100 µmol/L IBMX + 1 mmol/L ACh. Although cAMP levels were high in oocytes treated with 100 µmol/L IBMX, levels were reduced in groups treated simultaneously with 100 µmol/L ACh. Furthermore, compared with mature oocytes, ACh-treated GV oocytes exhibited significantly lower (by approximately 2.3-fold) or absent Ca(2+) peaks. 4. The results of the present study indicate that maturation of GV oocytes, arrested by IBMX treatment, is resumed following ACh treatment and that this effect is due to downregulation of cAMP rather than changes in intracellular Ca(2+) levels. |
| File Format | HTM / HTML |
| ISSN | 03051870 |
| Issue Number | 7 |
| Volume Number | 38 |
| e-ISSN | 14401681 |
| Journal | Clinical and Experimental Pharmacology and Physiology |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2011-07-01 |
| Publisher Place | Australia |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Physiology Discipline Pharmacology Acetylcholine Pharmacology Physiology Cyclic Amp Antagonists & Inhibitors Metabolism Oocytes Drug Effects 1-methyl-3-isobutylxanthine Animals Cells, Cultured Down-regulation Female Meiosis Mice Mice, Inbred Icr Cytology Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Physiology (medical) Pharmacology |
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