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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, L. X. Wang, S. Hou, J. Hu, R. L. An, X. R. |
| Description | Country affiliation: China Author Affiliation: Wang LX ( State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.) |
| Abstract | The objective of current study was to compare the nuclear configurations of canine oocytes recovered from between follicles after isolation. Follicles isolated were classified into follicle-S (follicles located in the ovarian surface) and follicle-I (follicles located inside the ovary) based on the follicle location in the ovary. Nuclear stages of canine oocytes recovered from follicle-S and follicle-I were examined by phase-contrast microscopy after isolation. Results demonstrated that canine GV stage oocytes can be classified into three types based on the status of the nuclear envelope, nucleolus, and chromatin: type A, type B, and type C. In follicle-S group, the majority (95.5%) of canine GV stage oocytes was of type B. All canine GV stage oocytes recovered from follicle-S (including type B and type C) were characterized by nuclear envelope disappearance prior to nucleolus collapse. In contrast, in follicle-I group, the majority (60.2%) of canine GV stage oocytes was of type C. Unexpectedly, a small proportion of canine GV stage oocytes from follicle-I (donated type A) were characterized by nuclear envelope disappearance following nucleolus collapse. In conclusion, nuclear configurations of each type of canine GV stage oocytes may differ from each other. Distributions of each type of canine GV stage oocytes may associate with the follicle location in the ovary. |
| File Format | HTM / HTML |
| ISSN | 09671994 |
| Issue Number | 1 |
| Volume Number | 19 |
| e-ISSN | 14698730 |
| Journal | Zygote |
| Language | English |
| Publisher | Cambridge University Press |
| Publisher Date | 2011-02-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Embryology Oocytes Ultrastructure Ovarian Follicle Ovary Animals Cell Nucleus Metabolism Cells, Cultured Dogs Female Microscopy, Phase-contrast Nuclear Envelope Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology |
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