NDLI: Effect of ambient light exposure of media and embryos on development and quality of porcine parthenogenetically activated embryos.

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Effect of ambient light exposure of media and embryos on development and quality of porcine parthenogenetically activated embryos.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, Rong Liu, Ying Pedersen, Hanne Skovsgaard Callesen, Henrik
Description	Country affiliation: Denmark Author Affiliation: Li R ( Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.); Liu Y ( Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.); Pedersen HS ( Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.); Callesen H ( Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.)
Abstract	Light exposure is a common stress factor during in vitro handling of oocytes and embryos that originates from both microscope and ambient light. In the current study, the effect of two types of ambient light (daylight and laboratory light) on porcine parthenogenetically activated (PA) embryos was tested in two experiments: (1) ambient light on medium subsequently used for embryo in vitro development; and (2) ambient light exposure on activated oocytes before in vitro development. The results from Experiment 1 showed that exposure of culture medium to both types of ambient light decreased the percentage of blastocysts that showed good morphology, only after 24 h exposure. The results from Experiment 2 revealed a reduction in both blastocyst formation and quality when activated oocytes were exposed to both types of ambient light. This effect was seen after only 1 h exposure and increased with time. In conclusion, exposure to ambient light can be harmful to embryo development, both when medium is exposed for a long period of time and, to a greater extent, when the embryo itself is exposed for >1 h. In practice, it is therefore recommended to protect both culture medium and porcine embryos against ambient light during in vitro handling in the laboratory.
File Format	HTM / HTML
ISSN	09671994
Issue Number	3
Volume Number	23
e-ISSN	14698730
Journal	Zygote
Language	English
Publisher	Cambridge University Press
Publisher Date	2015-06-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Embryology Blastocyst Physiology Embryo Culture Techniques Methods Oocytes Parthenogenesis Animals Apoptosis Culture Media Pharmacology Female In Vitro Oocyte Maturation Techniques Light Sus Scrofa Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology

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