NDLI: FACS selection of valuable mutant mouse round spermatids and strain rescue via round spermatid injection.

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FACS selection of valuable mutant mouse round spermatids and strain rescue via round spermatid injection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhu, Lian Zhou, Wei Kong, Peng-Cheng Wang, Mei-Shan Zhu, Yan Feng, Li-Xin Chen, Xue-Jin Jiang, Man-Xi
Description	Country affiliation: China Author Affiliation: Zhu L ( Department of Laboratory Animal Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China.); Zhou W ( Department of Developmental Biology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China.); Kong PC ( Department of Laboratory Animal Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China.); Wang MS ( Department of Laboratory Animal Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China.); Zhu Y ( Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Committee,Shanghai Institute of Planned Parenthood Research,Shanghai 200032,China.); Feng LX ( Department of Developmental Biology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China.); Chen XJ ( Department of Laboratory Animal Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China.); Jiang MX ( Department of Laboratory Animal Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China.)
Abstract	Round spermatid injection (ROSI) into mammalian oocytes can result in the development of viable embryos and offspring. One current limitation to this technique is the identification of suitable round spermatids. In the current paper, round spermatids were selected from testicular cells with phase contrast microscopy (PCM) and fluorescence-activated cell sorting (FACS), and ROSI was performed in two strains of mice. The rates of fertilization, embryonic development and offspring achieved were the same in all strains. Significantly, round spermatids selected by PCM and FACS were effectively used to rescue the infertile Pten-null mouse. The current results indicate that FACS selection of round spermatids can not only provide high-purity and viable round spermatids for use in ROSI, but also has no harmful effects on the developmental capacity of subsequently fertilized embryos. It was concluded that round spermatids selected by FACS are useful for mouse strain rederivation and rescue of infertile males; ROSI should be considered as a powerful addition to the armamentarium of assisted reproduction techniques applicable in the mouse.
File Format	HTM / HTML
ISSN	09671994
Issue Number	3
Volume Number	23
e-ISSN	14698730
Journal	Zygote
Language	English
Publisher	Cambridge University Press
Publisher Date	2015-06-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Embryology Flow Cytometry Methods Sperm Injections, Intracytoplasmic Spermatids Cytology Animals Embryo Transfer Female Male Mice, Inbred Icr Mice, Mutant Strains Mice, Transgenic Microscopy, Phase-contrast Pten Phosphohydrolase Genetics Pregnancy Pregnancy Rate Physiology Testis Journal Article
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology

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FACS selection of valuable mutant mouse round spermatids and strain rescue via round spermatid injection

Intracytoplasmic injection using spermatids and subsequent pregnancies: round versus elongated spermatids.

Production of fertile offspring from genetically infertile male mice.

Fourteen babies born after round spermatid injection into human oocytes.

The activity (calcium oscillator?) responsible for human oocyte activation after injection with round spermatids is associated with spermatid nuclei.

Porcine embryos produced after intracytoplasmic sperm injection using xenogeneic pig sperm from neonatal testis tissue grafted in mice.

Cytochalasin B treatment of mouse oocytes during intracytoplasmic sperm injection (ICSI) increases embryo survival without impairment of development.

Development of blastocyst-stage embryos after round spermatid injection in patients with complete spermiogenesis failure.

Derivation of sperm from xenografted testis cells and tissues of the peccary (Tayassu tajacu).

FACS selection of valuable mutant mouse round spermatids and strain rescue via round spermatid injection.

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FACS selection of valuable mutant mouse round spermatids and strain rescue via round spermatid injection

Intracytoplasmic injection using spermatids and subsequent pregnancies: round versus elongated spermatids.

Production of fertile offspring from genetically infertile male mice.

Fourteen babies born after round spermatid injection into human oocytes.

The activity (calcium oscillator?) responsible for human oocyte activation after injection with round spermatids is associated with spermatid nuclei.

Porcine embryos produced after intracytoplasmic sperm injection using xenogeneic pig sperm from neonatal testis tissue grafted in mice.

Cytochalasin B treatment of mouse oocytes during intracytoplasmic sperm injection (ICSI) increases embryo survival without impairment of development.

Development of blastocyst-stage embryos after round spermatid injection in patients with complete spermiogenesis failure.

Derivation of sperm from xenografted testis cells and tissues of the peccary (Tayassu tajacu).

FACS selection of valuable mutant mouse round spermatids and strain rescue via round spermatid injection.