NDLI: Continual expression throughout the cell cycle and downregulation upon adipogenic differentiation makes nucleostemin a vital human MSC proliferation marker.

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Year: 2011 Volume: 7 Issue: 1

Continual expression throughout the cell cycle and downregulation upon adipogenic differentiation makes nucleostemin a vital human MSC proliferation marker.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Oktar, Pinar Akpinar Yildirim, Sibel Balci, Deniz Can, Alp
Description	Country affiliation: Turkey Author Affiliation: Oktar PA ( Department of Histology and Embryology, Ankara University School of Medicine, Sihhiye, 06100, Ankara, Turkey.)
Abstract	Nucleostemin (NS) is a nucleolar protein expressed in stem and cancer cells. In combination with nuclear/nucleolar proteins, NS has been demonstrated to be involved in cell-cycle regulation and telomere maintenance. NS expression reflects the cell's proliferation state indicating that the cell is active in the cell cycle, whereas NS signals disappear upon differentiation. This study analyzes the spatio-temporal (nucleolar/nuclear localization during interphase and M-phase) NS remodeling in two distinct human mesenchymal stem cell (MSC) populations to discriminate the NS differences, if any, throughout their stem cell and differentiation states. Beside its prominent multilobular nucleolar localization in interphase cells, coexistence of NS with chromosome arms during mitosis was also observed. Disruption of mitotic microtubules induced dissociation of NS from the chromosome arms and scattered it into the cytoplasm. Compared to deciduous dental pulp MSCs, NS mRNA expression gradually decreased upon aging in umbilical cord stroma-derived MSCs as culture time increased. Following adipogenic differentiation of the latter, NS signals gradually disappeared in both dividing and non-dividing cells, even before the morphological and functional signs of adipogenic transformation appeared. Quantitative NS mRNA measurements showed that MSCs from two sources exhibit a strong nucleostemin expression similar to embryonic stem cells. In conclusion, apart from its novel chromosomal localization shown in this study, nucleolar NS can be considered as a marker that indicates the proliferation/differentiation states in human MSCs. Moreover, differences in the relative NS protein and mRNA levels may reflect the degree of proliferation and can be used to characterize in vitro expansion capabilities.
File Format	HTM / HTML
ISSN	15508943
Issue Number	2
Volume Number	7
e-ISSN	15586804
Journal	Stem Cell Reviews and Reports
Language	English
Publisher	Springer
Publisher Date	2011-06-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Cell Biology Adipocytes Metabolism Cell Cycle Down-regulation Gtp-binding Proteins Mesenchymal Stromal Cells Nuclear Proteins Cytology Adolescent Biological Markers Cell Differentiation Cell Nucleolus Cell Nucleus Size Cells, Cultured Chromosomes, Human Colchicine Pharmacology Dental Pulp Genetics Gene Expression Regulation Humans Male Mitosis Transcription, Genetic Tubulin Modulators Umbilical Cord Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine

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