| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Van Der Hoorn, Marie-louise P. Van Egmond, Angela Swings, Godelieve M. J. S. Van Beelen, Els Van Der Keur, Carin Tirado-González, Irene Blois, Sandra M. Karumanchi, S. Ananth Bianchi, Diana W. Claas, Frans H. J. Scherjon, Sicco A. |
| Description |
Author Affiliation: van der Hoorn ML ( Department of Obstetrics, Leiden University Medical Center, P.O. Box 9600 (location code: K6-26), 2300 RC Leiden, The Netherlands. Electronic address: m.l.p.van_der_hoorn@lumc.nl.); van Egmond A ( Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.); Swings GM ( Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.); van Beelen E ( Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.); van der Keur C ( Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.); Tirado-González I ( Charité Centrum 12 for Internal Medicine and Dermatology, Laboratory of Reproductive Medicine, University Medicine of Berlin, Campus Virchow Klinikum, Berlin, Germany.); Blois SM ( Charité Centrum 12 for Internal Medicine and Dermatology, Laboratory of Reproductive Medicine, University Medicine of Berlin, Campus Virchow Klinikum, Berlin, Germany.); Karumanchi SA ( Center for Vascular Biology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.); Bianchi DW ( Mother Infant Research Institute at Tufts Medical Center, Boston, MA, USA.); Claas FH ( Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.); Scherjon SA ( Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands) |
| Abstract |
In oocyte donation (OD) pregnancies, there is a higher level of antigenic dissimilarity between mother and fetus compared with naturally conceived (NC) pregnancies. We hypothesize that a higher degree and/or a different type of immunoregulation is necessary to maintain an uncomplicated OD pregnancy. Different immunological aspects of successful OD pregnancies (n=28) were compared with those of NC pregnancies (n=51), and non-donor IVF (n=20) pregnancies. Maternal peripheral blood mononuclear cells (mPBMCs) were characterized by flow cytometry; the outcome correlated with the number of mother-child HLA mismatches. The fetus-specific alloreactivity of mPBMCs was measured in a mixed lymphocyte reaction. The percentages of CD4(+)CD25(bright) and CD4(+)CD25(dim) cells were higher in mPBMCs of OD and IVF pregnancies compared with NC pregnancies. The percentage of CD4(+)CD25(dim) cells in mPBMCs of OD pregnancies correlated positively with the number of HLA mismatches. Functional studies showed a lower proliferative response to umbilical cord blood by mPBMCs in OD pregnancies. In conclusion, we found a higher degree of peripheral immunoregulation in OD and IVF pregnancies compared with NC pregnancies. A higher number of HLA mismatches in successful OD pregnancies leads to increased percentages of activated T cells in peripheral blood, but not to a higher alloreactivity to the fetus. These studies show that immunoregulation in OD pregnancy is different from that in NC pregnancies. The antigenic dissimilarity in OD pregnancies may play a role in the pathophysiology of preeclampsia. |
| File Format |
HTM / HTML |
| ISSN |
01650378 |
| Volume Number |
101-102 |
| e-ISSN |
18727603 |
| Journal |
Journal of Reproductive Immunology |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-03-01 |
| Publisher Place |
Ireland |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Immunology Discipline Reproductive Medicine Hla Antigens Immunology Isoantigens Oocyte Donation Oocytes T-lymphocytes, Regulatory Adult Antigens, Cd4 Metabolism Cell Proliferation Cells, Cultured Female Histocompatibility, Maternal-fetal Humans Immune Tolerance Immunomodulation Immunophenotyping Interleukin-2 Receptor Alpha Subunit Lymphocyte Activation Lymphocyte Culture Test, Mixed Middle Aged Pregnancy Comparative Study Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology Reproductive Medicine Obstetrics and Gynecology |
