| Author |
Carvalho, Aline de Souza da Silva, Márcia Vanusa Gomes, Francis Soares Paiva, Patrícia Maria Guedes Malafaia, Carolina Barbosa da Silva, Tulio Diego Vaz, Antônio Fernando de Melo da Silva, Alexandre Gomes Arruda, Isabel Renata de Souza Napoleão, Thiago Henrique Carneiro-da-Cunha, Maria das Graças Correia, Maria Tereza dos Santos |
| Description |
Country affiliation: Brazil Author Affiliation: Carvalho Ade S ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.); da Silva MV ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.); Gomes FS ( Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, 57072-900 Maceió, Alagoas, Brazil.); Paiva PM ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.); Malafaia CB ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.); da Silva TD ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.); Vaz AF ( Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande, Jatobá, 58700-970 Patos, Paraíba, Brazil.); da Silva AG ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.); Arruda IR ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.); Napoleão TH ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil. Electronic address: thiagohn86@yahoo.com.br.); Carneiro-da-Cunha Md ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.); Correia MT ( Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.) |
| Abstract |
Apuleia leiocarpa is a tree found in Caatinga that has great value in the timber industry. Lectins are carbohydrate-binding proteins with several biotechnological applications. This study shows the isolation, characterization, and antibacterial activity of A. leiocarpa seed lectin (ApulSL). The lectin was chromatographically isolated from a crude extract (in 150 mM NaCl) by using a chitin column. ApulSL adsorbed to the matrix and was eluted using 1.0 M acetic acid. Native ApulSL was characterized as a 55.8-kDa acidic protein. SDS-PAGE showed three polypeptide bands, whereas two-dimensional electrophoresis revealed four spots. The peptides detected by MALDI TOF/TOF did not show sufficient homology (<30%) with the database proteins. Circular dichroism spectroscopy suggested a disordered conformational structure, and fluorescence spectrum showed the presence of tyrosine residues in the hydrophobic core. The hemagglutinating activity of ApulSL was present even after heating to 100 °C, was Mn(2+)-dependent, and inhibited by N-acetylglucosamine, D(-)-arabinose, and azocasein. ApulSL demonstrated bacteriostatic and bactericide effects on gram-positive and gram-negative species, being more effective against three varieties of Xanthomonas campestris (MIC ranging from 11.2 to 22.5 µg/mL and MBC of 22.5 µg/mL). The results of this study reinforce the importance of biochemical prospecting of Caatinga by revealing the antibacterial potential of ApulSL. |
