NDLI: Development of an antigen-capture enzyme-linked immunosorbent assay using monoclonal antibodies for detecting H6 avian influenza viruses.

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Development of an antigen-capture enzyme-linked immunosorbent assay using monoclonal antibodies for detecting H6 avian influenza viruses.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, Yi-Tung Tsao, Zak Chang, Shu-Ting Juang, Ron-Huay Wang, Lih-Chiann Chang, Chung-Ming Wang, Ching-Ho
Description	Country affiliation: Taiwan Author Affiliation: Chen YT ( School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.)
Abstract	The H6 subtype of avian influenza virus (AIV) infection occurs frequently in wild and domestic birds. AIV antigen detection is preferred for controlling AIV as birds are infected before they produce antibodies. The purpose of this study was to develop an early diagnostic method for AIV detection. Six monoclonal antibodies (mAbs) developed from a field H6N1 AIV strain were tested for their ability to bind to viruses. The two that showed the greatest binding ability to AIVs were used for antigen detection. An antigen-capture enzyme-linked immunosorbent assay (ELISA) to detect H6 AIVs was developed using these mAbs. One mAb was coated onto an ELISA plate as the capture antibody. The other mAb was used as the detector antibody after labeling with horseradish peroxidase. The antigen-capture ELISA detected H6N1 AIVs but not H5 AIVs, human H1N1, H3N2 influenza or other viruses. This antigen-capture ELISA could be used to specifically detect H6N1 AIV.
File Format	HTM / HTML
ISSN	16841182
e-ISSN	19959133
Journal	Journal of Microbiology, Immunology and Infection
Issue Number	3
Volume Number	45
Language	English
Publisher	Elsevier
Publisher Date	2012-06-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Immunology Antibodies, Monoclonal Chemistry Antigens, Viral Enzyme-linked Immunosorbent Assay Influenza A Virus Isolation & Purification Influenza In Birds Virology Animals Immunology Metabolism Influenza A Virus, H5n2 Subtype Classification Diagnosis Limit Of Detection Trachea Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Immunology and Microbiology Immunology and Allergy Microbiology (medical)

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