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Evaluation of different serological tests for the detection of antibodies against highly pathogenic avian influenza in experimentally infected ostriches (Struthio camelus)

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Evaluation of different serological tests for the detection of antibodies against highly pathogenic avian influenza in experimentally infected ostriches (Struthio camelus)

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Toffan, Anna Olivier, Adriaan Mancin, Marzia Tuttoilmondo, Valentina Facco, Daniele Capua, Ilaria Terregino, Calogero
Description	Country affiliation: Italy Author Affiliation: Toffan A ( OIE, FAO and National Reference Laboratory for Avian Influenza and Newcastle Disease-Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Padova, Italy.)
Abstract	In the present study we collected 177 serum samples from ostriches (Struthio camelus) infected experimentally with A/ostrich/South Africa/Middleton/2004 (H5N2) highly pathogenic avian influenza virus. We tested these samples using the haemagglutination inhibition (HI) test, the agar gel immunodiffusion test and three enzyme-linked immunosorbent assay kits. We considered the HI test, with homologous antigen and including pre-treatment of sera with 10% chicken red blood cells, as the gold standard. Detectable specific antibodies appeared on day 7 post-infection and persisted until the termination of the experiment. The relative sensitivity and specificity of the tests under evaluation and Cohen's K value were calculated. The results reported herein could be of assistance to decision-makers in drafting guidelines for the definition of the health status of ostriches and for trade purposes.
File Format	HTM / HTML
ISSN	03079457
e-ISSN	14653338
DOI	10.1080/03079450903431390
Journal	Avian Pathology
Issue Number	1
Volume Number	39
Language	English
Publisher	Taylor & Francis
Publisher Date	2010-02-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Veterinary Medicine Discipline Zoology Antibodies, Viral Blood Influenza A Virus, H5n2 Subtype Immunology Influenza In Birds Diagnosis Struthioniformes Animals Enzyme-linked Immunosorbent Assay Hemagglutination Inhibition Tests Immunodiffusion Isolation & Purification Virology Sensitivity And Specificity Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Animal Science and Zoology Food Animals

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Evaluation of different serological tests for the detection of antibodies against highly pathogenic avian influenza in experimentally infected ostriches (Struthio camelus)