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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Magdalan, Jan Ostrowska, Alina Piotrowska, Aleksandra Gomulkiewicz, Agnieszka Podhorska-Okolów, Marzena Patrzalek, Dariusz Szelag, Adam Dziegiel, Piotr |
| Description | Country affiliation: Poland Author Affiliation: Magdalan J ( Department of Pharmacology, Wroclaw Medical University, Wroclaw, Poland.) |
| Abstract | Fatalities due to mushroom poisonings are increasing worldwide, with high mortality rate resulting from ingestion of amanitin-producing species. Intoxications caused by amanitin-containing mushrooms represent an unresolved problem in clinical toxicology since no specific and fully efficient antidote is available. The objective of this study was a comparative evaluation of benzylpenicillin (BPCN), acetylcysteine (ACC) and silibinin (SIL) as an antidotes in human hepatocytes intoxicated with alpha-amanitin (alpha-AMA). All experiments were performed on cultured human hepatocytes. Cytotoxicity evaluation of cultured cells using MTT assay and measurement of lactate dehydrogenase (LDH) activity was performed at 12, 24 and 48h of exposure to alpha-AMA and/or antidotes. The significant decline of cell viability and significant increase of LDH activity were observed in all experimental hepatocyte cultures after 12, 24 and 36h exposure to alpha-AMA at concentration 2microM. Exposure of the cells to alpha-AMA resulted also in significant reduction of cell spreading and attachment. However, addition of tested antidotes to experimental cultures significantly stimulated cell proliferation and attachment. In cell cultures exposed simultaneously to alpha-AMA and tested antidotes cytotoxic parameters (MTT and LDH) were not significantly different from control incidences. The cytoprotective effect of all antidotes was not dose-related, which reflects a high efficacy of all these substances. Administration of studied antidotes was not associated with any adverse effects in hepatocytes. The administration of ACC, BPCN or SIL to human hepatocyte cultures showed a similar strong protective effect against cell damage in alpha-AMA toxicity. |
| File Format | HTM / HTML |
| ISSN | 09402993 |
| Issue Number | 4 |
| Volume Number | 62 |
| e-ISSN | 16181433 |
| Journal | Experimental and Toxicologic Pathology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-07-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Toxicology Discipline Pathology Acetylcysteine Pharmacology Alpha-amanitin Toxicity Antidotes Hepatocytes Drug Effects Penicillin G Poisons Silymarin Cell Culture Techniques Cell Proliferation Cells, Cultured Cytoprotection Dose-response Relationship, Drug Humans Comparative Study Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Toxicology Pathology and Forensic Medicine |
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