NDLI: Semi-automated relative quantification of cell culture contamination with mycoplasma by Photoshop-based image analysis on immunofluorescence preparations.

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Semi-automated relative quantification of cell culture contamination with mycoplasma by Photoshop-based image analysis on immunofluorescence preparations.

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Semi-automated relative quantification of cell culture contamination with mycoplasma by Photoshop-based image analysis on immunofluorescence preparations.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kumar, Ashok Yerneni, Lakshmana K.
Description	Country affiliation: India Author Affiliation: Kumar A ( Cell Biology Laboratory, Institute of Pathology (ICMR), P.O. Box 4909, Safdarjung Hospital Campus, New Delhi 110029, India.)
Abstract	Mycoplasma contamination in cell culture is a serious setback for the cell-culturist. The experiments undertaken using contaminated cell cultures are known to yield unreliable or false results due to various morphological, biochemical and genetic effects. Earlier surveys revealed incidences of mycoplasma contamination in cell cultures to range from 15 to 80%. Out of a vast array of methods for detecting mycoplasma in cell culture, the cytological methods directly demonstrate the contaminating organism present in association with the cultured cells. In this investigation, we report the adoption of a cytological immunofluorescence assay (IFA), in an attempt to obtain a semi-automated relative quantification of contamination by employing the user-friendly Photoshop-based image analysis. The study performed on 77 cell cultures randomly collected from various laboratories revealed mycoplasma contamination in 18 cell cultures simultaneously by IFA and Hoechst DNA fluorochrome staining methods. It was observed that the Photoshop-based image analysis on IFA stained slides was very valuable as a sensitive tool in providing quantitative assessment on the extent of contamination both per se and in comparison to cellularity of cell cultures. The technique could be useful in estimating the efficacy of anti-mycoplasma agents during decontaminating measures.
File Format	HTM / HTML
ISSN	10451056
Issue Number	1
Volume Number	37
e-ISSN	10958320
Journal	Biologicals
Language	English
Publisher	Elsevier
Publisher Date	2009-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Immunology Automatic Data Processing Methods Equipment Contamination Image Processing, Computer-assisted Mycoplasma Cytology Software Animals Instrumentation Cell Culture Techniques Cells, Cultured Cercopithecus Aethiops Statistics & Numerical Data Fluorescent Antibody Technique Hela Cells Humans Macaca Mulatta Mice Isolation & Purification Vero Cells Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Pharmacology Bioengineering Applied Microbiology and Biotechnology Biotechnology

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