NDLI: Cross comparison of rapid mycoplasma detection platforms.

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Year: 2010 Volume: 38 Issue: 1

Year: 2009 Volume: 37

Year: 2008 Volume: 36

Year: 2007 Volume: 35

Cross comparison of rapid mycoplasma detection platforms.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lawrence, Bill Bashiri, Houman Dehghani, Houman
Description	Country affiliation: United States Author Affiliation: Lawrence B ( Biosafety Development Laboratory, Cellular Resources, Amgen, 1201 Amgen Court West, Seattle, WA 98119, USA. bill.lawrence@amgen.com)
Abstract	The use of animal and plant derived raw materials in mammalian cell culture processes may provide a possible route of entry for adventitious contaminants such as mycoplasma. Mycoplasma contaminations of cell culture represent a serious challenge to the production of biotechnology derived therapeutics. The slow growing nature of mycoplasma can disguise their infection of cultures since cells may continue to proliferate, though at reduced levels and with lesser output of engineered protein. Rapid identification of mycoplasma contaminated cell cultures and materials enables a faster response time to prevent the spread of the contamination. We describe here the comparison of different mycoplasma detection methods: two nucleic acid-based technologies, the standard mycoplasma culture procedure, and a hybrid culture-quantitative PCR assay. In this study, a cell line infected with two species of mycoplasma was used to compare the different detection methods. Our data demonstrates that the two nucleic acid-based techniques are robust methods for detection of mycoplasma and have similar detection capability. In contrast, no mycoplasma was detected in the standard culture assay or in a hybrid culture-quantitative PCR assay. This shows a potential limitation of the culture assay that relies on the ability of mycoplasma to grow in broth media.
File Format	HTM / HTML
ISSN	10451056
Issue Number	2
Volume Number	38
e-ISSN	10958320
Journal	Biologicals
Language	English
Publisher	Elsevier
Publisher Date	2010-03-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Immunology Bacteriological Techniques Instrumentation Methods Mycoplasma Isolation & Purification Animals Biosensing Techniques Cho Cells Cell Culture Techniques Cells, Cultured Cercopithecus Aethiops Cricetinae Cricetulus Dna, Bacterial Analysis Humans Models, Biological Classification Genetics Growth & Development Mycoplasma Infections Diagnosis Microbiology Sensitivity And Specificity Vero Cells Comparative Study Evaluation Studies Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine Pharmacology Bioengineering Applied Microbiology and Biotechnology Biotechnology

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