NDLI: Epigenetic Modulators Enhance Constitutive and Liver-Specific Reporter Expression in Murine Liver Progenitor Cell Lines.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Diepeveen, Luke Aris Watson, Michel Elyse McSpadden, Sarah Beth Strauss, Robyn Patricia Callus, Bernard Andrew Yeoh, George Cheng
Description	Country affiliation: Australia Author Affiliation: Diepeveen LA ( 1 The Centre for Medical Research, Harry Perkins Institute of Medical Research , Nedlands, WA, Australia .); Watson ME ( 1 The Centre for Medical Research, Harry Perkins Institute of Medical Research , Nedlands, WA, Australia .); McSpadden SB ( 2 Center for Cell Therapy and Regenerative Medicine, School of Medicine and Pharmacology, Crawley, WA, Australia .); Strauss RP ( 3 School of Chemistry and Biochemistry, University of Western Australia , Crawley, WA, Australia .); Callus BA ( 3 School of Chemistry and Biochemistry, University of Western Australia , Crawley, WA, Australia .); Yeoh GC ( 3 School of Chemistry and Biochemistry, University of Western Australia , Crawley, WA, Australia .)
Abstract	Stem cells expressing reporter constructs are extremely useful for their tracking in vivo or for determining cell lineage fate in vivo and in vitro. We generated liver progenitor cell (LPC) lines from actin-EGFP and TAT-GRE-lacZ mice. LPCs from the actin-EGFP mouse facilitate cell tracing following transplant as the reporter is constitutively expressed. LPCs from the TAT-GRE-lacZ mouse express ß-galactosidase under the control of the tyrosine aminotransferase (TAT) promoter and are only active in mature hepatocytes. We found that the utility of such LPC lines becomes severely limited by downregulation of transgene expression following extended culture. We show that epigenetic mechanisms are responsible for suppressing expression of both transgenes. Enhancement of transgene expression in both LPC lines was achieved by treating the cell lines with either the histone acetylating agent sodium butyrate or the DNA demethylating agent 5-azacytidine.
File Format	HTM / HTML
ISSN	21524947
Issue Number	10
Volume Number	21
e-ISSN	21524955
Journal	Tissue Engineering Part C: Methods
Language	English
Publisher	Mary Ann Liebert
Publisher Date	2015-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Cell Differentiation Epigenesis, Genetic Hepatocytes Metabolism Liver Stem Cells Animals Cell Line Genes, Reporter Cytology Mice Organ Specificity Tyrosine Transaminase Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

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