NDLI: Mesenchymal Stem Cells Derived from Peripheral Blood Retain Their Pluripotency, but Undergo Senescence During Long-Term Culture.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fu, Wei-Li Li, Jian Chen, Gang Li, Qi Tang, Xin Zhang, Cheng-Hao
Description	Author Affiliation: Fu WL ( Department of Orthopaedics, West China Hospital, Sichuan University , Chengdu, P.R. China .); Li J ( Department of Orthopaedics, West China Hospital, Sichuan University , Chengdu, P.R. China .); Chen G ( Department of Orthopaedics, West China Hospital, Sichuan University , Chengdu, P.R. China .); Li Q ( Department of Orthopaedics, West China Hospital, Sichuan University , Chengdu, P.R. China .); Tang X ( Department of Orthopaedics, West China Hospital, Sichuan University , Chengdu, P.R. China .); Zhang CH ( Department of Orthopaedics, West China Hospital, Sichuan University , Chengdu, P.R. China .)
Abstract	Peripheral blood-derived mesenchymal stem cells (PB-MSCs) show promise as a source of cells for autologous transplantation because they can be harvested through minimally invasive procedures. To ensure adequate numbers of cells for transplantation and tissue regeneration, PB-MSCs must first be cultured and expanded in vitro, but whether long-term passage modifies their properties has been poorly understood. In this study we triggered production of PB-MSCs in rabbits using granulocyte colony-stimulating factor (G-CSF) and AMD3100, and then isolated and expanded the cells in culture until they reached a state of senescence, usually after about 20 passages. Cultures of low-, middle-, and high-passage numbers were compared in terms of morphology, proliferative capacity, phenotype, differentiation potential, apoptosis, metabolic indicators, and senescence. As passage number increased, MSCs retained their elongated spindle shape, but became larger and flatter, slowed in growth gradually, and increased proportion of cells showed G1 arrest. The proportions of apoptotic cells, production of reactive oxygen species (ROS), and ADP/ATP ratio increased with passage number. Expression of senescence-associated ß-galactosidase increased, while telomerase activity decreased. On the other hand, cultures did not show significant changes in phenotype or lose their ability to differentiate into three lineages as passage number increased. These results suggest that PB-MSCs maintain their stem cell properties during prolonged culturing, but they undergo senescence that may be due to apoptosis and production of ROS. These findings may help to standardize in vitro production of PB-MSCs for tissue engineering.
File Format	HTM / HTML
ISSN	21524947
Issue Number	10
Volume Number	21
e-ISSN	21524955
Journal	Tissue Engineering Part C: Methods
Language	English
Publisher	Mary Ann Liebert
Publisher Date	2015-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Apoptosis Blood Cells Metabolism Cell Aging Cell Proliferation Mesenchymal Stromal Cells Animals Cytology Cell Culture Techniques Cells, Cultured Rabbits Time Factors Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in