NDLI: Magnetically Guided Fabrication of Multilayered Iron Oxide/Polycaprolactone/Gelatin Nanofibrous Structures for Tissue Engineering and Theranostic Application.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, Qingtao Ge, Liangpeng Wan, Wenbing Jiang, Junzi Zhong, Wen Ouyang, Jun Xing, Malcolm
Description	Country affiliation: China Author Affiliation: Li Q ( 1 Guangdong Provincial Medical Biomechanical Key Laboratory, Department of Anatomy, Southern Medical University , Guangzhou, China .); Ge L ( 2 Department of Mechanical Engineering, University of Manitoba , Winnipeg, Canada .); Wan W ( 3 Manitoba Institute of Child Health , Winnipeg, Canada .); Jiang J ( 2 Department of Mechanical Engineering, University of Manitoba , Winnipeg, Canada .); Zhong W ( 3 Manitoba Institute of Child Health , Winnipeg, Canada .); Ouyang J ( 4 Chongqing Academy of Animal Sciences , Chongqing, China .); Xing M ( 1 Guangdong Provincial Medical Biomechanical Key Laboratory, Department of Anatomy, Southern Medical University , Guangzhou, China .)
Abstract	A persistent challenge in tissue engineering is the fabrication of manipulatable scaffolds for implantation in clinical treatments and use in disease models for drug screening. Electrospinning of nanofibrous membranes is an emerging technology in artificial extracellular matrix (ECM) design that can offer precisely tunable microenvironments upon assembly into three-dimensional (3D) scaffolds that mimic the in vivo ECM structure. In this study, we report a facile and versatile strategy for preparing 3D multilayered constructs from Fe3O4/polycaprolactone (PCL)/gelatin nanofibrous membranes. This method combines membrane assembly with noncontact magnetic force to preserve the mechanical integrity and interconnectivity of the 3D scaffolds. An ordered layer structure can be achieved using a magnetic control technique through the addition of magnetic nanoparticles into the PCL/gelatin nanofibers. We first verified the magnetic properties and structures of magnetic nanofibers according to X-ray diffraction, hysteresis, scanning electron microscopy, and transmission electron microscopy. We tested the potential toxicity and osteogenic differentiation of mesenchymal stem cells seeded on the layered scaffolds. To add further functionality to the scaffolds, the membranes were coated with silver nanoparticles and shown to inhibit the growth of Escherichia coli and Staphylococcus aureus, which are responsible for most cases of infection-related implant failure. Finally, we tested the utility of magnetic membranes implanted in an animal model as a contrast agent for magnetic resonance imaging. Scaffolds formed using the presented magnetically guided fabrication strategy have the potential to mimic the structure and function of human tissues and also may be applied in disease models to study cell-cell interactions.
File Format	HTM / HTML
ISSN	21524947
Issue Number	10
Volume Number	21
e-ISSN	21524955
Journal	Tissue Engineering Part C: Methods
Language	English
Publisher	Mary Ann Liebert
Publisher Date	2015-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Ferric Compounds Chemistry Gelatin Magnetic Fields Mesenchymal Stromal Cells Metabolism Osteogenesis Polyesters Tissue Scaffolds Animals Extracellular Matrix Humans Cytology Mice Theranostic Nanomedicine Tissue Engineering Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in