NDLI: Construction and characterization of a sox9b transgenic reporter line.

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Construction and characterization of a sox9b transgenic reporter line.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Plavicki, Jessica S. Baker, Tracie R. Burns, Felipe R. Xiong, Kong M. Gooding, Alex J. Hofsteen, Peter Peterson, Richard E. Heideman, Warren
Description	Country affiliation: United States Author Affiliation: Plavicki JS ( School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA. plavicki@wisc.edu.)
Abstract	The transcription factor SOX9 is a member of the SRY-related high-mobility-group box (SOX) superfamily of genes. In mammals, Sox9 plays important roles in many developmental processes including craniofacial, skeletal and heart morphogenesis, retinal and brain development, and gonad differentiation. Human mutations in SOX9 or the SOX9 promoter result in campomelic dysplasia, a severe genetic disorder, which disrupts skeletal, craniofacial, cardiac, neural and reproductive development. Due to the duplication of the teleost fish genome, zebrafish (Danio rerio) have two Sox9 genes: sox9a and sox9b. Loss of sox9b in zebrafish results in loss of function phenotypes that are similar to those observed in humans and mice. In order to generate a transgenic sox9b:EGFP reporter line, we cloned a 2450 bp fragment of the sox9b promoter and fused it to an EGFP reporter. Consistent with reported sox9b expression and function, we observed sox9b:EGFP in the developing heart, skeletal and craniofacial structures, brain, retina, and ovaries. Our resulting transgenic line is a useful tool for identifying and studying sox9b function in development and visualizing a number of zebrafish organs and tissues in which sox9b is normally expressed.
File Format	HTM / HTML
ISSN	02146282
e-ISSN	16963547
DOI	10.1387/ijdb.140288jp
Journal	The International Journal of Developmental Biology
Issue Number	9
Volume Number	58
Language	English
Publisher	University of the Basque Country Press
Publisher Date	2014-01-01
Publisher Place	Spain
Access Restriction	Open
Subject Keyword	Discipline Embryology Discipline Biology Animals, Genetically Modified Growth & Development Embryo, Nonmammalian Cytology Metabolism Gene Expression Regulation, Developmental Green Fluorescent Proteins Sox9 Transcription Factor Zebrafish Proteins Zebrafish Animals Genetics Brain Embryology Craniofacial Abnormalities Heart Physiology Immunoenzyme Techniques In Situ Hybridization Mice Muscle, Skeletal Ovary Retina Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Embryology

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