NDLI: Comparison of a TaqMan real-time polymerase chain reaction assay with a loop-mediated isothermal amplification assay for detection of Gallid herpesvirus 1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ou, Shan-Chia Giambrone, Joseph J. Macklin, Kenneth S.
Description	Country affiliation: United States Author Affiliation: Ou SC ( Poultry Science Department, Auburn University, Auburn, AL 36849-5416, USA.)
Abstract	A TaqMan real-time polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) assay were developed to detect Gallid herpesvirus 1 (GaHV-1, formerly Infectious laryngotracheitis virus). The standard curve of real-time PCR was established, and the sensitivity reached 10 copies/µl. In the current study, the conversion between viral titer and GaHV-1 genomic copy number was constructed. Six primers for LAMP assay amplified target gene at 65°C within 45 min, and the detection limit was 60 copies/µl. The 6 primers were highly specific, sensitive, and reproducible for detection of GaHV-1. Although the sensitivity of LAMP was lower than that of real-time PCR, LAMP was faster, less expensive, and did not require a thermocycler. The LAMP assay would be a viable alternative assay in diagnostic laboratories that do not employ real-time PCR technology.
File Format	HTM / HTML
ISSN	10406387
Issue Number	1
Volume Number	24
e-ISSN	19434936
Journal	Journal of Veterinary Diagnostic Investigation
Language	English
Publisher	Sage Publications
Publisher Date	2012-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Veterinary Medicine Herpesviridae Infections Veterinary Herpesvirus 1, Gallid Genetics Nucleic Acid Amplification Techniques Poultry Diseases Virology Real-time Polymerase Chain Reaction Animals Chickens Diagnosis Methods Sensitivity And Specificity Comparative Study Journal Article
Content Type	Text
Resource Type	Article
Subject	Veterinary

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