NDLI: Discrimination between Mycoplasma and Acholeplasma species of bovine origin using digitonin disc diffusion assay, nisin disc diffusion assay, and conventional polymerase chain reaction.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Boonyayatra, Sukolrat Fox, Lawrence K. Gay, John M. Sawant, Ashish Besser, Thomas E.
Description	Country affiliation: United States Author Affiliation: Boonyayatra S ( Department of Veterinary Clinical Science, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA.)
Abstract	Microbiological culture of milk samples has been used as a standard diagnosis for Mycoplasma mastitis. This technique is effective in isolating mollicutes that are Mycoplasma-like; however, isolates may be misinterpreted as Acholeplasma species, which are indistinguishable from Mycoplasma species by culture. A study to contrast the abilities of 2 culture-based tests, digitonin and nisin disc diffusion assays and a conventional polymerase chain reaction (PCR) technique, to discriminate between Mycoplasma and Acholeplasma was performed using 16S ribosomal RNA gene partial sequencing as the gold standard of comparison. A total of 288 bovine mollicute field isolates (248 from milk and 40 from other organ sites) and 13 reference strains were tested. Results obtained from the digitonin disc diffusion assay when it was performed with all field isolates were 92.7% and 99.0% in agreement with the gold standard using 5 mm and 3 mm of zone of growth inhibition as thresholds, respectively. Considering only milk isolates, agreements between the digitonin disc diffusion assay with the gold standard were 97.2% and 100% using 5 mm and 3 mm of zone of growth inhibition as thresholds, respectively. Culture identification using the nisin disc diffusion assay and the PCR was in a 100% agreement with the gold standard. Comparable results using culture-based nisin and digitonin disc diffusion assays, and PCR, to distinguish Mycoplasma and Acholeplasma species was found, especially for isolates from bovine milk.
File Format	HTM / HTML
ISSN	10406387
Issue Number	1
Volume Number	24
e-ISSN	19434936
Journal	Journal of Veterinary Diagnostic Investigation
Language	English
Publisher	Sage Publications
Publisher Date	2012-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Veterinary Medicine Acholeplasma Cattle Diseases Microbiology Digitonin Disk Diffusion Antimicrobial Tests Veterinary Gram-negative Bacterial Infections Mycoplasma Infections Mycoplasma Nisin Polymerase Chain Reaction Genetics Acholeplasma Laidlawii Animals Cattle Diagnosis Methods Female Rna, Ribosomal, 16s Journal Article
Content Type	Text
Resource Type	Article
Subject	Veterinary

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