NDLI: Rapid genotyping assays for the 4-base pair deletion of canine MDR1/ABCB1 gene and low frequency of the mutant allele in Border Collie dogs.

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Rapid genotyping assays for the 4-base pair deletion of canine MDR1/ABCB1 gene and low frequency of the mutant allele in Border Collie dogs.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mizukami, Keijiro Chang, Hye-Sook Yabuki, Akira Kawamichi, Takuji Hossain, Mohammad A. Rahman, Mohammad M. Uddin, Mohammad M. Yamato, Osamu
Description	Country affiliation: Japan Author Affiliation: Mizukami K ( Laboratory of Clinical Pathology, Department of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima 890-0065, Japan.)
Abstract	P-glycoprotein, encoded by the MDR1 or ABCB1 gene, is an integral component of the blood-brain barrier as an efflux pump for xenobiotics crucial in limiting drug uptake into the central nervous system. Dogs homozygous for a 4-base pair deletion of the canine MDR1 gene show altered expression or function of P-glycoprotein, resulting in neurotoxicosis after administration of the substrate drugs. In the present study, the usefulness of microchip electrophoresis for genotyping assays detecting this deletion mutation was evaluated. Mutagenically separated polymerase chain reaction (MS-PCR) and real-time PCR assays were newly developed and evaluated. Furthermore, a genotyping survey was carried out in a population of Border Collies dogs in Japan to determine the allele frequency in this breed. Microchip electrophoresis showed advantages in detection sensitivity and time saving over other modes of electrophoresis. The MS-PCR assay clearly discriminated all genotypes. Real-time PCR assay was most suitable for a large-scale survey due to its high throughput and rapidity. The genotyping survey demonstrated that the carrier and mutant allele frequencies were 0.49% and 0.25%, respectively, suggesting that the mutant allele frequency in Border Collies is markedly low compared to that in the susceptible dog breeds such as rough and smooth Collies.
File Format	HTM / HTML
ISSN	10406387
Issue Number	1
Volume Number	24
e-ISSN	19434936
Journal	Journal of Veterinary Diagnostic Investigation
Language	English
Publisher	Sage Publications
Publisher Date	2012-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Veterinary Medicine Dog Diseases Genetics Gene Deletion Gene Frequency P-glycoprotein Animals Dogs Electrophoresis, Polyacrylamide Gel Veterinary Genotype Pedigree Real-time Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Veterinary

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