NDLI: Ghrelin in gestational diabetes: serum level and mRNA expression in fat and placental tissue.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Telejko, B. Kuzmicki, M. Zonenberg, A. Modzelewska, A. Niedziolko-Bagniuk, K. Ponurkiewicz, A. Wawrusiewicz-Kurylonek, N. Nikolajuk, A. Szamatowicz, J. Laudanski, P. Kretowski, A. Gorska, M.
Description	Author Affiliation: Telejko B ( Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15 - 276 Bia l ystok Poland. telejkob@poczta.onet.pl)
Abstract	OBJECTIVES: We studied the effect of an oral glucose load on circulating ghrelin, as well as ghrelin and ghrelin receptor (GHS-R1a) mRNA expression in subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT) and placental tissue from pregnant women with gestational diabetes (GDM) and normal glucose tolerance (NGT). METHODS: Plasma total ghrelin levels were measured in 58 patients with GDM and 61 women with NGT by radioimmunoassay. Ghrelin and GHS-R1a mRNA expression was studied in 16 subjects with GDM and 20 healthy pregnant women at term, using RT-PCR. RESULTS: Basal ghrelin concentrations and the maximal decrease in ghrelin levels after glucose load did not differ in the women with GDM and NGT (399.1 [299.6-563.3] pg/ml vs. 400.9 [302.3-475.8] pg/ml and 127.6 [23.1-213.1] pg/ml vs. 101.7 [44.0-217.6] pg/ml, respectively). Ghrelin mRNA expression in placental tissue was significantly higher in the subjects with GDM than in the healthy pregnant women (0.06 [0.03-0.07] AU vs. 0.02 [0.015-0.03 AU], p=0.02), whereas GHS-R1a mRNA expression in all three tissues studied did not differ between the two groups. Multiple regression analysis revealed that ghrelin mRNA expression in SAT was significantly predicted by serum insulin (beta=0.62, p=0.01), explaining 42% of its variability. CONCLUSIONS: Ghrelin mRNA expression in placental tissue was higher in the GDM than in NGT subjects, whereas no association between circulating ghrelin and GDM was observed.
File Format	HTM / HTML
ISSN	09477349
Issue Number	2
Volume Number	118
e-ISSN	14393646
Journal	Experimental and Clinical Endocrinology & Diabetes
Language	English
Publisher	Thieme
Publisher Date	2010-02-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Endocrinology Adipose Tissue Metabolism Diabetes, Gestational Ghrelin Placenta Drug Effects Adult Analysis Of Variance Blood Glucose Genetics Female Glucose Administration & Dosage Humans Insulin Resistance Pregnancy Rna, Messenger Radioimmunoassay Receptors, Ghrelin Regression Analysis Reverse Transcriptase Polymerase Chain Reaction Statistics, Nonparametric Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Endocrinology, Diabetes and Metabolism Internal Medicine Endocrinology

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