NDLI: Multiplex microarray ELISA versus classical ELISA, a comparison study of pollutant sensing for environmental analysis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Desmet, Cloé Blum, Loïc J. Marquette, Christophe A.
Description	Country affiliation: France Author Affiliation: Desmet C ( Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Lyon 1 - CNRS 5246 ICBMS, Bâtiment CPE - 43, bd du 11 novembre 1918, 69622 Villeurbanne, Cedex, France. christophe.marquette@univ-lyon1.fr.)
Abstract	The present study describes the development, optimization and performance comparison of three ELISAs and one multiplex immunoassay in a microarray format. The developed systems were dedicated to the detection of three different classes of pollutants (pesticide, explosive and toxin) in water. The characteristics and performances of these two types of assays were evaluated and compared, in order to verify that multiplex immunoassays can replace ELISA for multiple analyte sensing. 2,4-Dichlorophenoxyacetic acid, 2,4,6-trinitrotoluene and okadaic acid were chosen as model targets and were immobilized in classical microtiter plate wells or arrayed at the surface of a microarray integrated within a classical 96-well plate. Once optimized, the classical ELISAs and microarray-based ELISA performances were evaluated and compared in terms of limit of detection, IC50, linearity range and reproducibility. Classical ELISAs provided quite good sensitivity (limit of detection down to 10 µg L(-1)), but the multiplex immunoassay was proven to be more sensitive (limit of detection down to 0.01 µg L(-1)), more reproducible and an advantageous tool in terms of cost and time expenses. This multiplex tool was then used for the successful detection of the three target molecules in spiked water samples and achieved very promising recovery rates.
File Format	HTM / HTML
ISSN	20507887
Issue Number	10
Volume Number	15
e-ISSN	20507895
Journal	Environmental Science: Processes & Impacts
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2013-10-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Environmental Health Environmental Monitoring Methods Enzyme-linked Immunosorbent Assay Microarray Analysis Water Pollutants, Chemical Analysis 2,4-dichlorophenoxyacetic Acid Limit Of Detection Okadaic Acid Reference Standards Trinitrotoluene Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Public Health, Environmental and Occupational Health Management, Monitoring, Policy and Law

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