NDLI: Toxicological application of primary hepatocyte cell cultures of Atlantic cod (Gadus morhua)--effects of BNF, PCDD and Cd.

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Toxicological application of primary hepatocyte cell cultures of Atlantic cod (Gadus morhua)--effects of BNF, PCDD and Cd.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Søfteland, Liv Holen, Elisabeth Olsvik, Pål A.
Description	Country affiliation: Norway Author Affiliation: Søfteland L ( National Institute of Nutrition and Seafood Research, PO Box 2029 Nordnes, N-5817 Bergen, Norway. lso@nifes.no)
Abstract	Fish primary hepatocyte cultures are commonly used for toxicological assessment of contaminants. So far no one has described a protocol on how to use Atlantic cod hepatocytes in bioassays. In this work we describe an experiment in which we were able to isolate intact liver cells from mature individuals. Hepatic cytochrome P450 1A (CYP1A) expression in the isolated cells was evaluated with in situ hybridization after intraperitoneal injection with the strong CYP1A inducer ss-naphthoflavone (BNF). Cod hepatocytes were further exposed to 1,2,3,7,8-polychlorinated dibenzo-p-dioxin (PCDD) and cadmium (Cd). Transcriptional responses of 11 genes were quantified (CYP1A, metallothionein (MT), aryl hydrocarbon receptor 2 (AhR2), UDP-glucuronosyltransferase (UGT), glutathione S-transferase (GST), vitellogenin B (VTGB), hypoxia-inducible factor 1 (HIF1), heme oxygenase 1 (HO-1), transferrin, glutathione peroxidase (GPx) and heat shock protein 70 (HSP70)). Immunohistochemisty evaluation clearly showed elevated CYP1A mRNA expression in primary hepatocytes isolated from BNF-exposed fish. The transcriptional results showed that PCDD exposure resulted in a 311-fold up-regulation of CYP1A and Cd a 1.82-fold increase of MT. Unexpectedly, AhR2 and UGT mRNA levels were not significantly up-regulated in PCDD-exposed cod hepatocytes. HO-1 and transferrin showed a dose-dependent transcriptional response to Cd exposure. Cd appears to act as an endocrine-disrupting metal in exposed primary Atlantic cod hepatocytes. This study demonstrates the use of Atlantic cod primary hepatocyte cultures in toxicological research.
File Format	HTM / HTML
ISSN	15320456
Issue Number	4
Volume Number	151
Journal	Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Language	English
Publisher	Elsevier
Publisher Date	2010-05-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Toxicology Discipline Pharmacology Cadmium Chloride Toxicity Cytochrome P-450 Cyp1a1 Metabolism Gadus Morhua Physiology Hepatocytes Drug Effects Analogs & Derivatives Water Pollutants, Chemical Beta-naphthoflavone Animals Biological Markers Cells, Cultured Genetics Environmental Monitoring Enzyme Induction Female Gene Expression Regulation, Enzymologic Heme Oxygenase-1 Enzymology In Situ Hybridization Injections, Intraperitoneal Male Rna, Messenger Toxicity Tests Transferrin Up-regulation Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology Health, Toxicology and Mutagenesis Medicine Aquatic Science Biochemistry Toxicology Animal Science and Zoology

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Mass spectrometric analyses of microsomal cytochrome P450 isozymes isolated from ß-naphthoflavone-treated Atlantic cod (Gadus morhua) liver reveal insights into the cod CYPome.

Relationships between physiology, tissue contaminants, and biomarker responses in Atlantic cod (Gadus morhua L.).

Effects of water accommodated fractions of crude oils and diesel on a suite of biomarkers in Atlantic cod (Gadus morhua).

The influence of water temperature on induced liver EROD activity in Atlantic cod (Gadus morhua) exposed to crude oil and oil dispersants.

Kinetic properties of saxitoxin in Atlantic salmon (Salmo salar) and Atlantic cod (Gadus morhua).

Onboard Evaluation of Variable Water Flow and Recirculation Effects on Bleeding of Atlantic Cod (Gadus morhua)

Aryl hydrocarbon receptor protein and Cyp1A1 gene induction by LPS and phenanthrene in Atlantic cod (Gadus morhua) head kidney cells.

Experimental study of the susceptibility of Atlantic cod, Gadus morhua (L.), to infection with an IPNV strain pathogenic for Atlantic salmon, Salmo salar L.

Identification of transferrin in Atlantic cod Gadus morhua epidermal mucus.

Toxicological application of primary hepatocyte cell cultures of Atlantic cod (Gadus morhua)--effects of BNF, PCDD and Cd.

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Mass spectrometric analyses of microsomal cytochrome P450 isozymes isolated from ß-naphthoflavone-treated Atlantic cod (Gadus morhua) liver reveal insights into the cod CYPome.

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