| Author |
Dellai, Afef Dridi, Dorra Sakouhi, Seif Robert, Jacques Djelal, Hayet Mosrati, Ridha Cherif, Ameur Mansour, Hedi Ben |
| Description |
Author Affiliation: Dellai A ( Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie Laboratoire de Pharmacologie des Médicaments Anticancéreux, Institut Bergonie, Université Victor Segale); Dridi D ( Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie.); Sakouhi S ( Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie.); Robert J ( Laboratoire de Pharmacologie des Médicaments Anticancéreux, Institut Bergonie, Université Victor Segalen, Bordeaux, France.); Djelal H ( Ecole des Métiers de l'Environnement, Université Européenne de Bretagne, Rennes, France.); Mosrati R ( Laboratoire des Aliments Bioprocédés, Toxicologie, Environ-nements (ABTE-EA 4651), IUT de Caen, Université de Caen Basse-Normandie, Caen, France.); Cherif A ( Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie.); Mansour HB ( Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie Laboratoire de Pharmacologie des Médicaments Anticancéreux, Institut Bergonie, Université Victor Segale) |
| Abstract |
A bacterium was isolated from the river of Oued Hamdoun (Tunisia), and its phenotypic features, physiological and chemotaxonomic characteristics and phylogenetic analysis of 16S ribosomal RNA sequence revealed it as Pseudomonas peli (P. peli). Chlorpyrifos ethyl (CP) was used as the sole source of carbon and energy by P. peli, and it was cometabolised in the presence of glucose. CP was completely degraded by P. peli after 96 h of shake incubation. High-performance liquid chromatography analysis indicated that the biodegradation kinetics was not affected by the addition of glucose into the culture medium. In the present study, only transient accumulation of one major no-identified product was observed after 48 h of incubation, with no other persistent metabolites detected. Cytotoxicity of CP, before and after biodegradation with P. peli, was evaluated in vitro using the MTT-colorimetric assay against three human cancer cell lines (A549, lung cell carcinoma, HT29, colon adenocarcinoma and MCF7, breast adenocarcinoma). CP reduced viability of all human cell lines in a dose-dependent manner. Its activity was very remarkable against A549 cell line. However, cytotoxicity strongly decreased in CP obtained after incubation with P. peli Hence, we conclude that when incubated under appropriate conditions,P. peli has a metabolism that completely detoxifies CP. |
