NDLI: Dose-dependent genotoxicity of copper oxide nanoparticles stimulated by reactive oxygen species in human lung epithelial cells.

Please wait, while we are loading the content...

Anti-inflammatory Montelukast prevents toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin: Oxidative stress, histological alterations in liver, and serum cytokine levels.

The effect of di-n-butyl phthalate on testis and the potential protective effects of resveratrol.

Irreversible thyroid disruption induced after subchronic exposure to hexachlorobenzene in male rats.

A probable role of blood lead levels on some haematological parameters in traffic police, Lahore, Pakistan.

Chemotherapy-induced acral erythema with involvement of the face and neck: A case report.

Dose-dependent genotoxicity of copper oxide nanoparticles stimulated by reactive oxygen species in human lung epithelial cells.

Neuroprotective effects of folic acid on experimental diabetic peripheral neuropathy.

Phytochemical analysis, antibacterial, and antifungal assessment of aerial parts of Polygonatum verticillatum.

Changes of c-Myc and DNMT1 mRNA and protein levels in the rat livers induced by dibutyl phthalate treatment.

Protective effects of melatonin against arsenic-induced apoptosis and oxidative stress in rat testes.

In vivo antinociceptive and anticonvulsant activity of extracts of Heliotropium strigosum.

Enzymatic and histopathologic biomarkers in the flatfish Euryglossa orientalis from the northwestern Persian Gulf.

Hepatoprotective effect of Caesalpinia gilliesii and Cajanus cajan proteins against acetoaminophen overdose-induced hepatic damage.

Activation of Janus kinase/signal transducers and activators of transcription pathway involved in megakaryocyte proliferation induced by vanadium resembles some aspects of essential thrombocythemia.

The application of FLUENT in simulating outcomes from chlorine leakage accidents in a typical chemical factory.

Comparison between the effects of quercetin on seizure threshold in acute and chronic seizure models.

Evaluation of intravenous lipid emulsion on haloperidol-induced hypotension in rabbits.

Phyllanthin inhibits CCl4-mediated oxidative stress and hepatic fibrosis by down-regulating TNF- /NF-κB, and pro-fibrotic factor TGF-ß1 mediating inflammatory signaling.

Year: 2016 Volume: 32 Issue: 4

Year: 2016 Volume: 32 Issue: 3

Year: 2016 Volume: 32 Issue: 2

Year: 2016 Volume: 32 Issue: 1

Year: 2016

Year: 2015 Volume: 31

Year: 2014 Volume: 30

Year: 2013 Volume: 29

Year: 2012 Volume: 28

Year: 2011 Volume: 27

Year: 2010 Volume: 26

Year: 2009 Volume: 25

Year: 2008 Volume: 24

Year: 2006 Volume: 22

Year: 2002 Volume: 18

Dose-dependent genotoxicity of copper oxide nanoparticles stimulated by reactive oxygen species in human lung epithelial cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Akhtar, Mohammad Javed Kumar, Sudhir Alhadlaq, Hisham A. Alrokayan, Salman A. Abu-Salah, Khalid M. Ahamed, Maqusood
Description	Country affiliation: Saudi Arabia Author Affiliation: Akhtar MJ ( King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia.); Kumar S ( Department of Zoology, University of Lucknow, Lucknow, India.); Alhadlaq HA ( King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia.); Alrokayan SA ( Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.); Abu-Salah KM ( King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia.); Ahamed M ( King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia maqusood@gmail.com.)
Abstract	Copper oxide nanoparticles (CuO NPs) are of great interest in nanoscience and nanotechnology because of their broad industrial and commercial applications. Therefore, toxicity of CuO NPs needs to be thoroughly understood. The aim of this study was to investigate the cytotoxicity, genotoxicity, and oxidative stress induced by CuO NPs in human lung epithelial (A549) cells. CuO NPs were synthesized by solvothermal method and the size of NPs measured under transmission electron microscopy (TEM) was found to be around 23 nm. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and lactate dehydrogenase (LDH) assays showed that CuO NPs (5-15 µg/ml) exert cytotoxicity in A549 cells in a dose-dependent manner. Comet assay suggested concentration-dependent induction of DNA damage due to the exposure to CuO NPs. The comet tail moment was 27% at 15 µg/ml of CuO NPs, whereas it was 5% in control (p < 0.05). The flow cytometry data revealed that CuO NPs induced micronuclei (MN) in A549 cells dose dependently. The frequency of MN was 25/10(3) cells at 15 µg/ml of CuO NPs, whereas it was 2/10(3) cells for control. CuO NPs were also found to induce oxidative stress in a concentration-dependent manner, which was indicated by induction of reactive oxygen species (ROS) and lipid peroxidation along with glutathione depletion. Moreover, MN induction and DNA damage were significantly correlated with ROS (R(2) = 0.937 for ROS vs. olive tail moment, and R(2) = 0.944 for ROS vs. MN). Taken together, this study suggested that CuO NPs induce genotoxicity in A549 cells, which is likely to be mediated through ROS generation and oxidative stress.
File Format	HTM / HTML
ISSN	07482337
Issue Number	5
Journal	Toxicology and Industrial Health
Volume Number	32
e-ISSN	14770393
Language	English
Publisher	Sage Publications
Publisher Date	2016-05-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Occupational Medicine Discipline Toxicology
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Combined toxicity of amorphous silica nanoparticles and methylmercury to human lung epithelial cells.

Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in human lung cells

Cyto-genotoxicity of amphibole asbestos fibers in cultured human lung epithelial cell line: role of surface iron.

XRCC1 Deficiency Sensitizes Human Lung Epithelial Cells to Genotoxicity by Crocidolite Asbestos and Libby Amphibole

Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dusts

Toxic response of zinc oxide nanoparticles in human epidermal keratinocyte HaCaT cells

Iron oxide nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling

Calcium-dependent cyto- and genotoxicity of nickel metal and nickel oxide nanoparticles in human lung cells

Effects of zinc oxide nanoparticles on gene expression profile in human keratinocytes

Dose-dependent genotoxicity of copper oxide nanoparticles stimulated by reactive oxygen species in human lung epithelial cells.

Similar Documents

Combined toxicity of amorphous silica nanoparticles and methylmercury to human lung epithelial cells.

Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in human lung cells

Cyto-genotoxicity of amphibole asbestos fibers in cultured human lung epithelial cell line: role of surface iron.

XRCC1 Deficiency Sensitizes Human Lung Epithelial Cells to Genotoxicity by Crocidolite Asbestos and Libby Amphibole

Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dusts

Toxic response of zinc oxide nanoparticles in human epidermal keratinocyte HaCaT cells

Iron oxide nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling

Calcium-dependent cyto- and genotoxicity of nickel metal and nickel oxide nanoparticles in human lung cells

Effects of zinc oxide nanoparticles on gene expression profile in human keratinocytes

Dose-dependent genotoxicity of copper oxide nanoparticles stimulated by reactive oxygen species in human lung epithelial cells.