NDLI: High resolution melting curve analysis of DNA sequence alterations of various sizes.

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High resolution melting curve analysis of DNA sequence alterations of various sizes.

Year: 2010 Volume: 16 Issue: 2

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High resolution melting curve analysis of DNA sequence alterations of various sizes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Becságh, Péter Varga, Katalin Szakács, Orsolya Kopper, László Orosz, Zsolt
Description	Country affiliation: Hungary Author Affiliation: Becságh P ( Roche Hungary Ltd, Budaörs, Hungary. peter.becsagh@roche.com)
Abstract	In the diagnostic workflow we need to think in algorithms, containing more assays. One of the most important task in the management of cancer patient is to detect nucleic acid sequence changes in clinical specimens. Before using the most expensive method to analyze direct our targets, a screening assay is needed to reduce the number of samples. In the detection of gene-sequence alterations classical screening methods are available, as SSCP, DGGE or TGGE, (Finke Exp Clin Endocrinol Diabetes 104:92-97, 1996; Lessa and Applebaum Mol Ecol 2:119-129, 1993) however these are very time consuming processes. At this time in the molecular lab the real-time PCR equipments are very popular and with the function of melting curve analysis it can be a very convenient, simple and cost-efficient screening method.
File Format	HTM / HTML
ISSN	12194956
Issue Number	3
Volume Number	16
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2010-09-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Dna Mutational Analysis Methods Gastrointestinal Stromal Tumors Genetics Proto-oncogene Proteins C-kit Reverse Transcriptase Polymerase Chain Reaction Algorithms Base Sequence Dna Primers Freezing Humans Molecular Sequence Data Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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