NDLI: The Probable Role of Tumor Stem Cells for Lymph Node Metastasis in Supraglottic Carcinoma

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lu, Sumei Tian, Jiajun Lv, Zhenghua Wang, Haibo Bai, Xiaohui Liu, Wenwen Li, Jianfeng Xu, Wei
Description	Author Affiliation: Lu S ( Institute of Eye and ENT, Provincial Hospital affiliated to Shandong University, Jinan, 250021, People's Republic of China.)
Abstract	Tumor stem cells (TSC), which are considered as likely candidates for the origin of cancer, are deduced to be responsible for tumor metastasis theoretically. We therefore investigated whether TSC were associated with lymph node metastasis in supraglottic carcinoma. Immunohistochemistry was performed for CD44, CD133, and LYVE-1 to detect TSC and lymphatic vessel density (LVD) in 66 primary supraglottic carcinoma tissue samples from 30 patients with lymph node metastasis (N+) and 36 patients without (N0). Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of CD44 and CD133 at mRNA and protein levels in N+ and N0 primary tumors. The LVD was 22.4 ± 10.26 in 30N+ and 6.8 ± 4.09 in 36N0 samples subjected to immunohistochemistry, which was associated with their clinical nodal stages. There were 43.33% CD44-positive and 93.33% CD133-positive samples in 30N+, and 13.89% CD44-positive and 44.44% CD133-positive samples in 36N0 (P < 0.05). However, in each positive slide, there were only 5∼10% CD44-positive cells, but 70∼85% CD133-possitive cells. The expressions of CD44 and CD133 of N+ obtained through RT-PCR and Western blot were significantly higher than those of N0. These results suggest that TSC identified through CD44-positive cells in N+ were significantly higher than those in N0, indicating that TSC may be responsible for lymph node metastasis. CD133, whose expression is not restricted to TSC, may be unspecific for TSC identification in hypostatic supraglottic carcinoma.
File Format	HTM / HTML
ISSN	12194956
Issue Number	1
Volume Number	17
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2011-03-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Carcinoma Pathology Laryngeal Neoplasms Lymphatic Metastasis Neoplastic Stem Cells Adult Aged Antigens, Cd Analysis Biosynthesis Antigens, Cd44 Tumor Markers, Biological Blotting, Western Metabolism Female Glycoproteins Humans Immunohistochemistry Male Middle Aged Neoplasm Staging Peptides Reverse Transcriptase Polymerase Chain Reaction Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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