NDLI: miR-135a/b modulate cisplatin resistance of human lung cancer cell line by targeting MCL1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhou, Li Qiu, Tianzhu Xu, Jing Wang, Tongshan Wang, Jian Zhou, Xin Huang, Zebo Zhu, Wei Shu, Yongqian Liu, Ping
Description	Country affiliation: China Author Affiliation: Zhou L ( Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.)
Abstract	microRNAs (miRNAs) are short non-coding RNA molecules, which post-transcriptionally regulate genes expression and play crucial roles in diverse biological processes, such as development, differentiation, apoptosis, and proliferation. Here, we investigated the possible role of miRNAs in the development of drug resistance in human lung cancer cell line. We found that miR-135a/b were downregulated while MCL1 was upregulated in A549/CDDP (cisplatin) cells, compared with the parental A549 cells. In vitro drug sensitivity assay demonstrated that overexpression of miR-135a/b sensitized A549/CDDP cells to cisplatin. The luciferase activity of MCL1 3'-untranslated region-based reporter constructed in A549/CDDP cells suggested that MCL1 was the direct target gene of miR-135a/b. Enforced miR-135a/b expression reduced MCL1 protein level and sensitized A549/CDDP cells to CDDP-induced apoptosis. Taken together, our findings first suggested that hsa-miR-135a/b could play a role in the development of CDDP resistance in lung cancer cell line at least in part by modulation of apoptosis via targeting MCL1.
File Format	HTM / HTML
ISSN	12194956
Issue Number	4
Volume Number	19
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2013-10-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Antineoplastic Agents Pharmacology Cisplatin Lung Neoplasms Drug Therapy Metabolism Micrornas Myeloid Cell Leukemia Sequence 1 Protein Apoptosis Drug Effects Cell Line, Tumor Down-regulation Drug Resistance, Neoplasm Humans Genetics Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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