NDLI: Both butyrate incubation and hypoxia upregulate genes involved in the ruminal transport of SCFA and their metabolites

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Both butyrate incubation and hypoxia upregulate genes involved in the ruminal transport of SCFA and their metabolites

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dengler, F. Rackwitz, R. Benesch, F. Pfannkuche, H. Gäbel, G.
Description	Country affiliation: Germany Author Affiliation: Dengler F ( Institute of Veterinary Physiology, University of Leipzig, Leipzig, Germany.)
Abstract	Butyrate modulates the differentiation, proliferation and gene expression profiles of various cell types. Ruminal epithelium is exposed to a high intraluminal concentration and inflow of n-butyrate. We aimed to investigate the influence of n-butyrate on the mRNA expression of proteins involved in the transmembranal transfer of n-butyrate metabolites and short-chain fatty acids in ruminal epithelium. N-butyrate-induced changes were compared with the effects of hypoxia because metabolite accumulation after O2 depletion is at least partly comparable to the accumulation of metabolites after n-butyrate exposure. Furthermore, in various tissues, O2 depletion modulates the expression of transport proteins that are also involved in the extrusion of metabolites derived from n-butyrate breakdown in ruminal epithelium. Sheep ruminal epithelia mounted in Ussing chambers were exposed to 50 mM n-butyrate or incubated under hypoxic conditions for 6 h. Electrophysiological measurements showed hypoxia-induced damage in the epithelia. The mRNA expression levels of monocarboxylate transporters (MCT) 1 and 4, anion exchanger (AE) 2, downregulated in adenoma (DRA), putative anion transporter (PAT) 1 and glucose transporter (GLUT) 1 were assessed by RT-qPCR. We also examined the mRNA expression of nuclear factor (NF) κB, cyclooxygenase (COX) 2, hypoxia-inducible factor (HIF) 1 and acyl-CoA oxidase (ACO) to elucidate the possible signalling pathways involved in the modulation of gene expression. The mRNA expression levels of MCT 1, MCT 4, GLUT 1, HIF 1 and COX 2 were upregulated after both n-butyrate exposure and hypoxia. ACO and PAT 1 were upregulated only after n-butyrate incubation. Upregulation of both MCT isoforms and NFκB after n-butyrate incubation could be detected on protein level as well. Our study suggests key roles for MCT 1 and 4 in the adaptation to an increased intracellular load of metabolites, whereas an involvement of PAT 1 in the transport of n-butyrate also seems possible.
File Format	HTM / HTML
ISSN	09312439
Issue Number	2
Volume Number	99
e-ISSN	14390396
Journal	Journal of Animal Physiology and Animal Nutrition
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2015-04-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Physiology Discipline Veterinary Medicine Discipline Nutritional Sciences Butyric Acid Pharmacology Fatty Acids, Volatile Metabolism Gene Expression Regulation Drug Effects Oxygen Rumen Sheep Physiology Animals Biological Transport Female Administration & Dosage Rna, Messenger Genetics Tissue Culture Techniques Up-regulation Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

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Bicarbonate-dependent transport of acetate and butyrate across the basolateral membrane of sheep rumen epithelium.

Determination of volatile fatty acids clearance in intact ruminal digesta

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Corrigendum: The Regulation of Ruminal Short-Chain Fatty Acids on the Functions of Rumen Barriers

Epithelial capacity for apical uptake of short chain fatty acids is a key determinant for intraruminal pH and the susceptibility to subacute ruminal acidosis in sheep.

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Effect of feeding Lagerstroemia speciosa and conventional fodder based rations on nutrient utilization, ruminal metabolites and body weight gain in mithun (Bos frontalis).

Both butyrate incubation and hypoxia upregulate genes involved in the ruminal transport of SCFA and their metabolites

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Both butyrate incubation and hypoxia upregulate genes involved in the ruminal transport of SCFA and their metabolites