NDLI: Gallium-Loaded Dissolvable Microfilm Constructs that Provide Sustained Release of $Ga^{3+}$ for Management of Biofilms

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Herron, Maggie Schurr, Michael J. Murphy, Christopher J. McAnulty, Jonathan F. Czuprynski, Charles J. Abbott, Nicholas L.
Description	Country affiliation: United States Author Affiliation: Herron M ( Department of Chemical and Biological Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, WI, 53706, USA.); Schurr MJ ( Department of Surgery, School of Medicine, University of Colorado-Denver, 12631 E. 17th Avenue, Aurora, CO, 80045, USA.); Murphy CJ ( Department of Ophthalmology and Vision Sciences, School of Medicine, Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, 1423 Tupper Hall, Davis, CA, 95616, USA.); McAnulty JF ( Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015, Linden Dr, Madison, WI, 53706, USA.); Czuprynski CJ ( Department of Pathobiology, School of Veterinary Medicine, University of Wisconsin-Madison, 2015, Linden Dr, Madison, WI, 53706, USA.); Abbott NL ( Department of Chemical and Biological Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, WI, 53706, USA.)
Abstract	The persistence of bacterial biofilms in chronic wounds delays wound healing. Although $Ga^{3+}$ can inhibit or kill biofilms, precipitation as $Ga(OH)_{3}$ has prevented its use as a topical wound treatment. The design of a microfilm construct comprising a polyelectrolyte film that releases noncytotoxic concentrations of $Ga^{3+}$ over 20 d and a dissolvable micrometer-thick film of polyvinylalcohol that enables facile transfer onto biomedically important surfaces is reported. By using infrared spectroscopy, it is shown that the density of free carboxylate/carboxylic acid and amine groups within the polyelectrolyte film regulates the capacity of the construct to be loaded with $Ga^{3+}$ and that the density of covalent cross-links introduced into the polyelectrolyte film (amide-bonds) controls the release rate of $Ga^{3+}.$ Following transfer onto the wound-contact surface of a biologic wound dressing, an optimized construct is demonstrated to release ≈0.7 μg $cm^{−2}$ $d^{−1}$ of $Ga^{3+}$ over 3 weeks, thus continuously replacing $Ga^{3+}$ lost to precipitation. The optimized construct inhibits formation of P. aeruginosa (two strains; ATCC 27853 and PA01) biofilms for up to 4 d and causes pre-existing biofilms to disperse. Overall, this study provides designs of polymeric constructs that permit facile modification of the wound-contacting surfaces of dressings and biomaterials to manage biofilms.
File Format	HTM / HTML
ISSN	21922640
Issue Number	18
Volume Number	4
e-ISSN	21922659
Journal	Advanced Healthcare Materials
Language	English
Publisher	Wiley-VCH
Publisher Date	2015-12-30
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biomedical Engineering Biofilms Drug Effects Gallium Pharmacology Bandages Biomass Delayed-action Preparations Electrolytes Chemistry Kinetics Polyvinyl Alcohol Pseudomonas Aeruginosa Physiology Solubility Journal Article Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biomedical Engineering Pharmaceutical Science

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