NDLI: In vitro flow-through assay for rapid detection of endotoxin in human sera: A proof-of-concept.

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In vitro flow-through assay for rapid detection of endotoxin in human sera: A proof-of-concept.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kalita, Prasanta Chaturvedula, Lakshmi M. Sritharan, Venkataraman Gupta, Shalini
Description	Country affiliation: India Author Affiliation: Kalita P ( Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, India.); Chaturvedula LM ( Molecular Diagnostics & Biomarkers Laboratory, Global Medical Education and Research Foundation, Hyderabad, India.); Sritharan V ( Molecular Diagnostics & Biomarkers Laboratory, Global Medical Education and Research Foundation, Hyderabad, India.); Gupta S ( Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, India. Electronic address: shalinig@chemical.iitd.ac.in.)
Abstract	An increase in endotoxin concentration in the bloodstream can trigger activation of innate immune response leading to septic shock. There is currently no method available for rapid endotoxin detection at a patient's bedside. We demonstrate a simple, portable and cost-effective strategy to measure endotoxin levels in human serum within 5min using a flow-through assay. A drop of serum containing LPS was spotted on an endotoxin-affinity membrane placed over high-wicking absorbent pads. Subsequent addition of polymyxin B sulfate drug-conjugated gold nanoparticles allowed concentration-dependent visualization of spots by the naked eye in the clinically-relevant range of 10pg/mL to 10ng/mL. The results were quantified using a concentration-calibrated color chart and the assay performance was tested with archival plasma samples of 18 known septicemia patients. The results showed a reasonably good correlation with the patients' hematological data. This proof-of-concept study puts forth an interesting alternative for early septicemia diagnosis in future.
File Format	HTM / HTML
ISSN	15499634
Journal	Nanomedicine: Nanotechnology, Biology and Medicine
e-ISSN	15499642
Language	English
Publisher	Elsevier
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Nanotechnology Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Molecular Medicine Bioengineering Biomedical Engineering Pharmaceutical Science

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