NDLI: Increased toll-like receptor 9 expression is associated with the severity of paraquat-induced lung injury in mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Qian, J. Liu, L. Chen, L. Lu, X. Zhu, C.
Description	Country affiliation: China Author Affiliation: Qian J ( Department of Emergency Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.); Liu L ( Department of Emergency Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China liuli_renji@126.com.); Chen L ( Department of Emergency Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.); Lu X ( Department of Emergency Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.); Zhu C ( Department of Emergency Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.)
Abstract	BACKGROUND: Toll-like receptor 9 (TLR9), an important component of the innate immune system, contributes to the pathogenesis of lung injury. However, its role in paraquat (PQ)-induced lung injury has not been studied. METHOD: Mice were divided into PQ group (n = 24) and control group (n = 12). Mice in both groups were killed on either day 7 or day 28 after PQ (40 mg/kg intraperitoneally) or saline administration. TLR9 expression was evaluated through real-time polymerase chain reaction and immunohistochemistry. Concurrently, histopathological examinations of lung tissues were performed. On day 7, the lung weight to body weight ratios (LW/BW), total protein content, and inflammatory cytokine levels in bronchoalveolar lavage fluid (BALF) were measured. On day 28, fibrogenic cytokine expressions in lung tissues were measured. The correlations between TLR9 messenger RNA (mRNA) expression and different indicators of lung injury were then evaluated. RESULTS: TLR9 expression was significantly increased over time in lung tissues after PQ poisoning. On day 7, TLR9 expression increased in parallel with LW/BW ratio (r = 0.403, p < 0.05), BALF protein content (r = 0.706, p<0.01), and BALF inflammatory cytokine levels (interleukin 6 (IL-6): r = 0.619, IL-1ß: r = 0.930, tumor necrosis factor : r = 0.589, all p < 0.05). On day 28, elevated TLR9 expression was closely correlated with Ashcroft score (r = 0.726, p < 0.01), mRNA expressions of -smooth muscle actin, type I collagen and type III collagen (r = 0.926, 0.957, and 0.924, respectively; all p < 0.01). CONCLUSIONS: The TLR9 expression in lung tissue is markedly elevated during PQ-induced acute lung injury and pulmonary fibrosis and positively correlated with the severity of lung injury in mice.
File Format	HTM / HTML
ISSN	09603271
Issue Number	4
Volume Number	34
e-ISSN	14770903
Journal	Human & Experimental Toxicology
Language	English
Publisher	Sage Publication
Publisher Date	2015-04-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Toxicology Acute Lung Injury Immunology Herbicides Toxicity Paraquat Pulmonary Edema Toll-like Receptor 9 Chemically Induced Pathology Animals Bronchoalveolar Lavage Fluid Chemistry Cytokines Fibrosis Lung Mice, Inbred C57bl Rna, Messenger Metabolism Severity Of Illness Index Genetics Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology

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