NDLI: Production of staphylococcal enterotoxin R by Staphylococcus aureus strains.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lis, Elzbieta Podkowik, Magdalena Schubert, Justyna Bystron, Jaroslaw Stefaniak, Tadeusz Bania, Jacek
Description	Country affiliation: Poland Author Affiliation: Lis E ( Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.)
Abstract	Staphylococcal enterotoxin D and R (SED, SER) production was determined in 24 S. aureus strains harboring sed gene. Seven of them were not able to produce SED as evidenced by enzyme-linked immunosorbent assay and Western blotting. Sequencing revealed that all these strains harbor a variant of sed gene. Expression of SER was detectable in 22 out of 24 isolates, with variance in productivity ranging from â¼40 to 450 ng/mL. Out of the seven isolates not able to produce SED, three produced high amounts of SER (249-396 ng/mL), two produced less than 200 ng/mL of SER, and two were found to express no detectable amount of SER. Three of those were assigned to spa type t1677 with two being of agr type III and one of agr type I. One strain was t084, agr type II, one t603, agr type II, one 2920, agr type III, one t2920, agr type III, and one t5160, agr type I. Because conventional screening procedures involve only the detection of classical enterotoxins in food, the isolates not able to produce SED presented in this study could pose a threat to human health due to SER production.
File Format	HTM / HTML
ISSN	15353141
Issue Number	8
Volume Number	9
e-ISSN	15567125
Journal	Foodborne Pathogens and Disease
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2012-08-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Microbiology Discipline Parasitology Discipline Nutritional Sciences Enterotoxins Biosynthesis Genetics Staphylococcus Aureus Metabolism Bacterial Toxins Blotting, Western Cloning, Molecular Dna, Bacterial Enzyme-linked Immunosorbent Assay Food Contamination Analysis Food Microbiology Sequence Analysis, Dna Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Microbiology Applied Microbiology and Biotechnology Food Science

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