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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Maojun Meng, Fanliang Cao, Fangfang Qiao, Bo Liu, Guodong Liu, Hongying Zhou, Yizhuang Dong, Haiyan Gu, Yixin Xiao, Di Zhang, Yongchan Zhang, Jianzhong |
| Description | Country affiliation: China Author Affiliation: Zhang M ( National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. zhangmaojun@icdc.cn) |
| Abstract | Fourteen Campylobacter jejuni genes--porA, cadF, omp18, dnaK, flaC, peb1, peb2, peb3, peb4, ahpC, groEL, tuF, hipO, and Cj0069--were cloned and expressed in Escherichia coli BL21. The recombinant proteins were purified on histidine (His) and glutathione S-transferase (GST) trap columns using the ÄKTA Explorer 100 System. Recombinant proteins were visualized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The antigenicities of these recombinant proteins were assessed by Western blotting and enzyme-linked immunosorbent assays with anti-C. jejuni immune rabbit sera. Four recombinant proteins, including rGST-PorA, rHis-CadF, rGST-GroEL, and rGST-TuF, demonstrated reactions with both anti-serum and preimmune serum, while rHis-DnaK, rGST-FlaC, rGST-PEB2, rGST-PEB3, rGST-PEB4, and rGST-HipO showed variable antigenicity characteristics to the anti-sera derived from different C. jejuni strains. rHis-Omp18, rHis-PEB1, and rGST-AhpC demonstrated universal and specific antigenities with the entire anti-sera panel tested in this present study, while recombinant rGST-Cj0069 and rHis-DnaK did not react with any of the anti-C. jejuni sera tested. In conclusion, rGST-AhpC may be useful as a potential serodiagnostic antigen for C. jejuni infection. |
| File Format | HTM / HTML |
| ISSN | 15353141 |
| Issue Number | 8 |
| Volume Number | 9 |
| e-ISSN | 15567125 |
| Journal | Foodborne Pathogens and Disease |
| Language | English |
| Publisher | Mary Ann Liebert, Inc. |
| Publisher Date | 2012-08-01 |
| Publisher Place | United States |
| Access Restriction | Subscribed |
| Subject Keyword | Discipline Microbiology Discipline Parasitology Discipline Nutritional Sciences Antigens, Bacterial Immunology Bacterial Proteins Genetics Campylobacter Jejuni Amino Acid Sequence Animals Blotting, Western Campylobacter Infections Diagnosis Cloning, Molecular Electrophoresis, Polyacrylamide Gel Enzyme-linked Immunosorbent Assay Escherichia Coli Gene Expression Regulation, Bacterial Immune Sera Molecular Sequence Data Rabbits Recombinant Proteins Sodium Dodecyl Sulfate Metabolism Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Animal Science and Zoology Microbiology Applied Microbiology and Biotechnology Food Science |
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