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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Ming-xue Zhang, Yu-mei Esther, Jun He, Wei Che, Hong-hua Gu, Ping |
| Description | Country affiliation: China Author Affiliation: Zhang MX ( Department of Traditional Chinese Medicine, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, China. zhmx6228@163.com) |
| Abstract | OBJECTIVE: To study the effects of yiqi huoxue recipe and Coxsackie B virus type 3 (CVB3) on the expression of ribosomal protein S20 in rat cardiac myocytes, to explore the pathogenesis of myocarditis induced by CVB3 and the mechanism of yiqi huoxue recipe on gene level, and to further investigate whether yiqi huoxue recipe is an effective prescription for CVB3 myocarditis. METHODS: A modified suppression subtractive hybridization (SSH) was used to isolate differentially expressed genes between the CVB3 infection group and the treatment group with yiqi huoxue recipe. The results were further verified by fluorescence RT-PCR. RESULTS: The results of SSH showed that the gene expression of ribosomal protein S20 in the treatment group was higher than that in the CVB3 infection group (P<0.05), which agreed with the results of fluorescent RT-PCR. CONCLUSION: Down-regulation of ribosomal protein S20 mRNA expression might be one of the mechanisms in CVB3 myocarditis, and yiqi huoxue recipe could achieve the treatment of viral myocarditis by regulating the expression of ribosomal protein S20. |
| File Format | HTM / HTML |
| ISSN | 16720415 |
| Issue Number | 5 |
| Volume Number | 17 |
| e-ISSN | 19930402 |
| Journal | Chinese Journal of Integrative Medicine |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2011-05-01 |
| Publisher Place | China |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Therapeutics Drugs, Chinese Herbal Pharmacology Enterovirus B, Human Drug Effects Metabolism Myocytes, Cardiac Virology Ribosomal Proteins Animals Coxsackievirus Infections Drug Therapy Therapeutic Use Gene Expression Regulation Nucleic Acid Hybridization Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Genetics Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Complementary and Alternative Medicine |
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